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Updated: Jan 16, 2026

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia
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In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia

Published on: September 12, 2025

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在Paramcium tetraurelia中进行蛋白质相互作用研究的in vivo近距离生物化

Bozhidar-Adrian Stefanov1, Robin Hogg2, Dimitris Themis3

  • 1Institute of Cell Biology, University of Bern; adrian.stefanov@unibe.ch.

Journal of visualized experiments : JoVE
|September 29, 2025
PubMed
概括

研究人员开发了一种新的近距离生物化方法,用于研究Paramecium中的蛋白质相互作用. 这项技术使用工程DNA来标记蛋白质,有助于理解基因组编辑机制.

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Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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