暂时的受体潜在化物4调节基质刚度机械感知和跨细胞孔形成在人类的施莱姆运河细胞
Haiyan Li1, Cydney Wong2, Seyed Mohammad Siadat2
1Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, United States; Department of Ophthalmology, Emory University, Atlanta, GA, United States.
Acta biomaterialia
|September 29, 2025
概括
施莱姆运河细胞中的过渡受体潜在化物4 (TRPV4) 通道调节细胞硬性和毛孔形成,影响眼睛高血压. 向TRPV4可能通过恢复正常细胞机械生物学来提供新的青光眼治疗策略.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 眼科医生 眼科 眼科
背景情况:
- 施莱姆运河 (SC) 细胞的病理变化,如硬化和拉伸,与眼睛高血压和玻璃眼有关.
- 暂时受体潜在化物4 (TRPV4) 通道是细胞伸展激活的机械敏感离子通道,但它们在SC细胞机械生物学中的作用尚不清楚.
研究的目的:
- 研究TRPV4在SC细胞机理生物学中的作用及其作为眼睛高血压治疗点的潜力.
- 确定TRPV4活性如何影响SC细胞对基质度的反应,并影响细胞度和跨细胞孔形成.
主要方法:
- 使用组织模拟水凝来模拟SC细胞环境.
- 采用药理调制来抑制和激活TRPV4通道.
- 评估了actin细胞骨架,细胞外基质,细胞刚性和细胞间孔形成的变化.
主要成果:
- 持续的TRPV4抑制调节了基质刚度机械感知,降低了SC细胞刚度并增加了跨细胞孔形成.
- 急性TRPV4激活增加了的流入和跨细胞孔形成.
- 玻璃眼性SC细胞表现出TRPV4机械感知和跨细胞孔形成能力降低.
结论:
- TRPV4作为SC细胞中的关键机械传感器,调节细胞骨动力学,ECM重塑,细胞刚性和孔隙形成,这对于眼内压力调节至关重要.
- 在玻璃眼性SC细胞中减少TRPV4机械感知有助于减少跨细胞孔形成.
- 向SC细胞中的TRPV4为眼睛高血压提供了潜在的治疗策略,但干预措施可能需要针对疾病的具体定制.
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