相关实验视频

Updated: Jan 16, 2026

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Published on: February 3, 2020

病原体效应因子BcSSP2抑制了NPC相位分离,以促进Botrytis cinerea感染

Jiaojiao Wang^1,2, Dewei Wu^3,4, Gaofeng Pei⁵

¹College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China. nxywjj@163.com.

Nature communications

|September 29, 2025

概括

病原体作用者可以通过破坏生物分子相分离来抑制植物免疫力. 一种Botrytis cinerea效应剂,BcSSP2,向核孔综合体 (NPC),抑制宿主防御并促进感染.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

生物分子相位分离对于植物和动物免疫是至关重要的.
病原体作用者旨在克服宿主防御,以成功感染.
众所周知,很少有植物病原体影响者能够准相位分离介导免疫.

研究的目的:

通过破坏相分离来抑制植物免疫力的病原体效应因子的识别和表征.
调查这种效应器干扰宿主防御通路的机制.
探索这种策略在病原体中的进化保存.

主要方法:

来自 Botrytis cinerea 的效应蛋白 BcSSP2 的鉴定.
研究BcSSP2与植物核孔综合体 (NPC) 的相互作用.
分析BcSSP2对NUP62相隔离和MPK3核传输的影响.
在其他死菌虫中对BcSSP2同类的比较分析.

主要成果:

BcSSP2, Botrytis cinerea 的一个效应体,破坏了 NUP62 在植物 NPC 的相分离.
这种干扰抑制了免疫调节器MPK3的核运输,抑制了植物的防御.
BcSSP2同类物在死菌性菌体中广泛存在,并且也破坏了NUP62相分离.

结论:

破坏植物NPC相位分离是一种由死菌病原体使用的进化保守策略.
BcSSP2通过NPC相分离干扰抑制宿主免疫力来促进Botrytis cinerea感染.
这项研究揭示了一种新的病原体毒性机制,针对宿主相隔离动态.

更多相关视频

Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

Identification of Host Pathways Targeted by Bacterial Effector Proteins using Yeast Toxicity and Suppressor Screens

Identification of Host Pathways Targeted by Bacterial Effector Proteins using Yeast Toxicity and Suppressor Screens

Published on: October 25, 2019

相关概念视频

Gene Regulation During Sporulation

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...

Separation of Sister Chromatids

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...