一个新生儿衍生的单克隆IgM抗体选择性地调节肠道中的微生物代谢
Zihan He1, Shijie Gong1, Fan Mu1
1The Center for Microbes, Development and Health, Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai, China.
Nature communications
|September 29, 2025
概括
新生儿的IgM抗体可以塑造肠道细菌的新陈代谢. 一个发达的单克隆IgM (M291) 调节了小鼠的宿主和微生物代谢,而没有改变肠道微生物群的组成.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 代谢学 代谢学 代谢学
背景情况:
- 肠道微生物群和新陈代谢对婴儿的健康和发育至关重要.
- 母乳IgA抗体塑造婴儿的肠道微生物群,但非母乳养的婴儿具有不同的抗体配置.
- 新生儿IgM抗体可以识别并影响细菌的行为和新陈代谢.
研究的目的:
- 研究新生儿IgM抗体在调节肠道微生物群代谢中的作用.
- 开发一种单克隆IgM抗体 (M291),作为模仿新生儿天然抗体的工具.
- 在小鼠模型中评估M291对宿主和微生物代谢的体内影响.
主要方法:
- 新生儿IgM抗体与细菌相互作用的特征 in vitro.
- 从新生B细胞中开发出单克隆IgM抗体 (M291).
- 口服M291给没有细菌的小鼠,这些小鼠被定义的细菌群体或婴儿肠道微生物群所定居.
- 分析肠道中的代谢和转录变化.
主要成果:
- 新生儿IgM抗体可以改变细菌聚类和碳源利用.
- 口服M291调节了小鼠的代谢组和细菌转录组.
- 微生物的丰富性和多样性在M291治疗后基本保持不变.
- M291影响了宿主脂质代谢和胆酸分泌.
结论:
- 新生儿衍生抗体可以被设计为治疗工具,以调节肠道微生物和宿主代谢.
- 单克隆IgM M291显示出有针对性的代谢干预的潜力,而不会破坏微生物群的组成.
- 这种方法为通过基于抗体的调制来管理婴儿代谢健康提供了一种新的策略.
相关概念视频
01:22Development of Immunocompetence
790
The initiation of cell-mediated immunity can be observed as early as the third month of fetal growth, with active antibody-mediated immunity following approximately one month later.
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
790
01:15Transcytosis of IgG
4.0K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
4.0K
01:23Anatomy of the Intestines
86.8K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
86.8K