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中国软海Pelodiscus sinensis的染色体级基因组组合

Jing Chen¹, Jiayun Yao¹, Xuemei Yuan¹

¹Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Key Laboratory of Fishery Environment and Aquatic Product Quality and Safety of Huzhou City, Zhejiang Institute of Freshwater Fisheries, Huzhou, 313001, China.

Scientific data

|September 29, 2025

概括

这项研究为中国软海 (Pelodiscus sinensis) 提供了高质量的基因组组. 这种宝贵的资源有助于保护这一重要的水产养殖物种的遗传多样性.

科学领域:

水产养殖的基因组学
进行比较的基因组学.
保护遗传学保护遗传学

背景情况:

在亚洲水产养殖中,Pelodiscus sinensis因其营养和药用价值至关重要.
种繁殖的快速发展导致了显著的生殖质降解和种群混合.
迫切需要基因组资源来应对保护和繁殖的挑战.

研究的目的:

为了生成一个高质量的基因组组装Pelodiscus sinensis.
为胚胎细胞保护和选择性繁殖计划提供基础资源.
促进未来对P. sinensis.重要的特征遗传基础的研究.

主要方法:

使用了PacBio的HiFi,Illumina,Hi-C和RNA测序数据.
采用先进的基因组组装算法用于连接和支架的生成.
定组装了33个染色体的序列,并进行了基因预测和功能注释.

主要成果:

生成了一个2.24 Gb的基因组组件,具有高度 (107.61 Mb) 和脚手架 (129.58 Mb) N50值.
成功将95.42%的组件 (2.14 Gb) 定在33个染色体上.
预测了21532个编码蛋白质的基因,其中21149个功能性注释.

结论:

高质量的Pelodiscus sinensis基因组组合是该物种的重大进步.
这种基因组资源将有助于保护生殖质和改进育种策略.
促进基因研究和P. sinensis水产养殖的可持续发展.

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