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Chroococcus turigidus是一种新型的蓝菌源,用于l-酸酶的产生

Reham Gamal¹, Esmail M El-Fakharany², Khaled N M Elsayed³

¹National Institute of Oceanography and Fisheries, NIOF, Cairo, Egypt.

Scientific reports

|September 30, 2025

概括

海洋微藻Chroococcus turigidus是L-asparaginase的新型来源,L-asparaginase是一种具有抗白血病和抗癌性能的酶. 这项研究强调了其在治疗应用和食品生产方面的潜力.

科学领域:

生物技术是生物技术.
海洋生物学海洋生物学
酶学是一种酶学.

背景情况:

对于治疗白血病和减少食物中的烯胺,L-氨酸酶至关重要.
海洋微藻提供生物活性化合物的可持续来源.
烯胺是一种在食品中发现的致癌化合物.

研究的目的:

为了确定能够产生L-asparaginase的海洋微藻.
为了评估来自Chroococcus turigidus的L-asparaginase的抗癌活性.
评估 Chroococcus turigidus 对大规模酶生产的潜力.

主要方法:

隔离和选七种海洋微藻菌株的L-asparaginase活性.
通过产量最高的隔离物量化L-阿斯巴拉金酶的产量.
对乳腺癌 (MDA) 和肝癌 (HepG-2) 细胞系的藻类提取物细胞毒性的体外评估,与正常的人类皮肤纤维细胞 (HSF) 作为对照.

主要成果:

鉴定出Chroococcus turigidus是L-阿斯巴拉金酶的最高生产者 (212.413 IU/ml).
藻类提取物对HepG-2和MDA细胞系表现出显著的抗癌活性.
较低的IC50值 (126.3和169.8微克/毫升) 和选择性指数 (6.69和4.97) 表明与正常细胞相比,针对癌细胞的向毒性.

结论:

Chroococcus turigidus 是一个有前途的来源,用于生产L-asparaginase.
该酶表现出强大的抗癌性质,特别是对抗肝瘤细胞.
这种微藻适用于工业规模的生物质和酶生产,在医学和食品安全方面具有双重优势.

关键词:

这是一种抗癌药物.亚斯巴拉基纳酶是一种亚斯巴拉基纳酶.这种病原体是Chroococcus turigidus.海洋微藻是一种微藻.

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