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外源性塑料应力:聚烯诱导的表型变化和氧化应力适应在Pseudomonas aeruginosa中

Wenjing Zhang¹, Runcheng Zhou¹, Jingwei Pan¹

¹Department of Clinical Laboratory Technology, School of Medicine, Foshan University, Foshan, 528225, Guangdong, China.

Antonie van Leeuwenhoek

|September 30, 2025

概括

聚烯暴露会增加Pseudomonas aeruginosa (PA) 对抗生素的耐药性,并增强生物膜的形成. 这项研究揭示了PP如何影响PAPA.

科学领域:

微生物学微生物学
环境科学环境科学
材料科学材料科学材料科学

背景情况:

聚烯 (PP) 广泛用于医疗保健和实验室.
伪omonas aeruginosa (PA) 是一种机会性病原体.
了解PP对PA的影响对于感染控制至关重要.

研究的目的:

调查暴露于PP的PA中的表型和氧化应激变化.
为了确定短期PP暴露对PA毒性因子的影响.
探索PP和PA之间的分子相互作用.

主要方法:

在24小时内,将PA暴露在不同的PP度 (0,10,1000毫克/升) 中.
对抗生素耐药性的评估,生物膜的形成,运动性和皮约素的产生.
测量氧化应激标志物 (H2O2,麦隆迪甲基,谷氨).
对PP-PA受体相互作用的分子动力学模拟.

主要成果:

暴露在PP中增强了PA对西普罗夫洛克萨,伊米佩内姆,阿米卡和热纳米辛的耐药性.
观察到生物膜形成,皮约素生产和运动性的显著增加.
氧化应激标志物 (H2O2,) 增加,同时谷氨酸水平也上升.
分子动力学表明PP与PA LasR受体形成稳定的复合体.

结论:

短期的PP暴露会导致PA显著的表型变化和氧化应激.
PP压力增强PA的毒性,可能增加感染风险.
与LasR的PP相互作用可能会调解这些观察到的变化.
调查结果为公众健康策略提供信息,以防止在与PP相关的环境中发生PA感染.

关键词:

伪omonas aeruginosa 这种类型的病毒.抗生素耐药性抗生素耐药性氧化应激反应氧化应激反应现象类型的现象类型的聚烯聚烯的使用方法

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