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脱落CD4⁺T细胞的LAG-3棕化诱导功能障碍与重复的怀孕流产有关

Liyuan Cui¹, Fengrun Sun¹, Xinhang Meng¹

¹Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, 200090, P.R. China, 128 Shenyang Road.

Molecular medicine (Cambridge, Mass.)

|September 30, 2025

概括

复发性怀孕流产 (RPL) 与较低水平的淋巴细胞激活基因-3 (LAG-3) 阳性结晶性CD4+ T细胞有关,这些细胞通过抗炎性细胞因子的产生来维持怀孕至关重要.

科学领域:

免疫学免疫学免疫学
生殖生物学生殖生物学
细胞生物学细胞生物学

背景情况:

经常性流产 (RPL) 显著影响女性的健康,但其机制尚不清楚.
淋巴细胞激活基因-3 (LAG-3) 是一个关键的免疫检查点,调节免疫反应.
确定RPL的生物标志物对于早期诊断和干预至关重要.

研究的目的:

调查LAG-3表达在正常和复发性妊娠流产中CD4+ T细胞的作用.
探索LAG-3作为RPL的诊断生物标志物的潜力.

主要方法:

通过流细胞计和飞行时间的细胞计来分析LAG-3表达在状CD4+T细胞上.
在特定的T细胞子集中测量了细胞因子的产生.
进行了对公开可用的单细胞RNA测序数据的分析.
在体外和体内实验中,研究了棕细胞代谢的作用.

主要成果:

在正常怀孕中观察到更高的LAG-3+脱落CD4+T细胞的频率,产生抗炎细胞因子.
与流产相关联的LAG-3+阴茎CD4+T细胞的减少.
在RPL中,LAG-3RNA水平稳定,但LAG-3+细胞的棕化增加.
抑制棕代谢可以提高LAG-3的表达.

结论:

LAG-3在调节叶CD4+T细胞功能和维持正常怀孕方面发挥着至关重要的作用.
降低LAG-3表达在脱落的CD4+T细胞上可能作为RPL诊断的潜在生物标志物.
棕化在RPL的背景下影响LAG-3表达.

关键词:

果的CD4+T细胞现场行动组-3 棕甲基基化是棕甲基化的一种.怀孕怀孕怀孕怀孕经常发生的怀孕流产.

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