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从下生物膜中分离出来的Streptococcus hominis的完整基因组序列

Seok Bin Yang^1,2, Doyun Ku^1,2, Ji-Hoi Moon¹

¹Department of Oral Microbiology, College of Dentistry, Kyung Hee University, Seoul, 02447, Republic of Korea.

BMC genomic data

|September 30, 2025

概括

这项研究首次展示了口腔微生物组中Streptococcus hominis的高质量基因组. 新的基因组为口腔细菌及其适应机制提供了关键的见解.

科学领域:

微生物学微生物学
基因组学就是基因组学.
口腔微生物组研究

背景情况:

Streptococcus hominis 是最近发现的一种物种,其基因组数据有限,特别是来自口腔环境.
之前的研究缺乏对S. hominis的全面基因组信息,阻碍了对其在口腔微生物组中的作用的理解.

研究的目的:

测序和分析从人类下膜生物膜中分离的KHUD_010菌株Streptococcus hominis的完整基因组.
扩大S. hominis的基因组资源,并促进对口腔微生物组组成和功能的研究.

主要方法:

基因组DNA提取和16S rRNA基因测序用于物种确认.
使用PacBio Sequel II平台和de novo组装进行全基因组测序.
基因组注释和对比基因组学与现有的S. hominis菌株.

主要成果:

组装了一个高质量的单圆形染色体基因组 S. hominis KHUD_010 (1.88 Mbp, 39.04% GC含量).
标注揭示了1,793个蛋白质编码基因,4个rRNA操作子和120个tRNA,完整度为99.1%.
对比基因组学确定了核心,可用和菌株特定的基因群,包括KHUD_010.0中的18个独特群.

结论:

S. hominis KHUD_010 基因组代表了该物种基因组数据的显著扩展.
这种参考基因组将有助于未来对口腔微生物群多样性,宿主微生物相互作用和细菌适应性的研究.

关键词:

人类类链球菌 (Streptococcus hominis) 是一种细菌.基因组基因组基因组基因组基因组下生物膜是一种下生物膜.

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