超排卵改变了小鼠卵细胞中的端粒长度和端粒酶成分水平
Betul Tire1, Gunel Talibova1, Yesim Bilmez1
1Department of Histology and Embryology, Akdeniz University School of Medicine, Campus, Antalya, 07070, Türkiye.
Journal of ovarian research
|September 30, 2025
概括
超排卵是辅助生殖技术的一个关键步骤,通过改变端粒长度和端粒酶基因表达,可以对卵细胞质量产生负面影响. 这些变化可能会导致卵细胞成熟和胚胎早期发育期间的不良影响.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 辅助生殖技术 (ART) 使用受控的卵巢刺激 (超排卵) 来提高卵细胞产量.
- 过度排卵会导致不良影响,包括氧化应激,卵细胞质量降低和线粒体功能障碍.
- 氧化应激和线粒体功能障碍与端粒长度的变化有关.
研究的目的:
- 研究单次和重复超排卵对小鼠卵细胞中端粒长度和端粒酶成分表达的影响.
- 评估超排卵,端粒动态和荷尔蒙形状之间的关系.
主要方法:
- 在小鼠中使用淋巴激素诱导过度排卵.
- 在卵细胞中分析了端粒长度,端粒酶RNA成分 (Terc) 和端粒酶逆转录酶 (Tert) 的mRNA和蛋白质水平.
- 测量了雌激素,孕激素和氧化标志物的血清水平.
主要成果:
- 超排卵显著改变了小鼠卵细胞中的端粒长度.
- 端粒酶成分 (TERT蛋白,Terc,Tert mRNA) 的表达受到超排卵的影响.
- 荷尔蒙特征 (雌激醇,孕) 和氧化标志物发生变化,可能会影响端粒动态.
结论:
- 超排卵会影响卵细胞中的端粒长度和端粒酶表达,可能是通过荷尔蒙变化介导的.
- 变化的端粒长度和端粒酶活性可能会导致超排卵对卵细胞成熟和早期胚胎发育的不良影响.
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