Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

AutoBlot: deterministic single-cell western blotting reveals proteomic diversity in rare cell populations.

bioRxiv : the preprint server for biology·2026
Same author

Deterministic DNA barcoding using vacuum-driven loading of free oligonucleotides to microwell arrays.

bioRxiv : the preprint server for biology·2025
Same author

EpiBlot: Joint Mapping of Chromatin Accessibility and Targeted Proteomics in HER2-expressing Breast Cancer Systems.

bioRxiv : the preprint server for biology·2025
Same author

Single-cell western blotting of cytoplasmic cytokeratin 8 proteoforms.

The Analyst·2025
Same author

Paired Analyses of Nuclear Protein Targets and Genomic DNA by Single-Cell Western Blot and Single-Cell PCR.

Analytical chemistry·2025
Same author

Paired Analyses of Nuclear Protein Targets and Genomic DNA by Single-Cell Western Blot and Single-Cell PCR.

bioRxiv : the preprint server for biology·2025

相关实验视频

Updated: Jun 3, 2026

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
06:00

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

5.1K

用细胞索引提取单细胞核的方法

Trinh Lam1, Ana Esmeralda Gomez Martinez1, Alison Su1

  • 1Department of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|September 30, 2025
PubMed
概括

一个新的微流体装置,VacTrap,从哺乳动物细胞中分离单个细胞核,保留它们的空间起源,用于多组学研究. 这项技术克服了批量分离的局限性,使单细胞的详细分析成为可能.

关键词:
这种水凝是水凝.微流体学 在微流体学方面多种多种多种多种多种多种多种多种多种多种.有机器人 有机器人蛋白质组学是什么意思一个单细胞的单细胞.

更多相关视频

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
09:09

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Published on: December 9, 2022

7.0K
Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
07:12

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Published on: July 28, 2023

5.0K

相关实验视频

Last Updated: Jun 3, 2026

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
06:00

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

5.1K
Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
09:09

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Published on: December 9, 2022

7.0K
Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
07:12

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Published on: July 28, 2023

5.0K

科学领域:

  • 生物技术是生物技术.
  • 细胞生物学 细胞生物学
  • 微流体学 微流体学

背景情况:

  • 大量有机体分离掩盖了细胞间的关键异质性.
  • 现有的微流体技术难以将分离的有机细胞与其母细胞连接起来,从而实现多组学.

研究的目的:

  • 开发一种高通量微流体装置,用于从哺乳动物细胞中分离和空间索引单个核.
  • 通过保留核和它们的细胞起源之间的连接,使可靠的多组学读数成为可能.

主要方法:

  • "VacTrap"装置采用了三层系统:一个带有陷门底座的Bis-gel微波层,一个聚甲基 (PDMS) 微波层和一个真空分流器.
  • 通过微分洗剂分化 (DDF) 的细胞溶解释放核,而细胞质蛋白质被保留在Bis-gel中.
  • 真空辅助的陷门溶解与98%的效率同步核转移到PDMS层.

主要成果:

  • 从单个细胞核中成功分离细胞质蛋白质是通过in situ免疫检测验证的.
  • 形态分析证实了隔离后完整的核特征的保存.
  • VacTrap设备实现了98%的传输效率,保持了核完整性和空间索引.

结论:

  • VacTrap提供了一个强大的,自动化的平台,用于单细胞制备在多组应用.
  • 该设备有效地隔离核,同时保留空间信息,解决批量分成的局限性.
  • 这项技术通过实现精确的细胞器与细胞的重新连接,促进了先进的单细胞多组学.