Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

9.3K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Chemical basis of fluorogenic substrate probe design: revisiting the past to discover the new in biochemistry.

RSC chemical biology·2026
Same author

Solid-Phase Synthesis of ProTide Fluorogenic Probes Enables Systematic Profiling of Carboxypeptidase Activity.

Journal of the American Chemical Society·2026
Same author

A novel selective stabilizer of the ryanodine receptor 2 prevents stress-induced ventricular arrhythmias without impairing cardiac function.

British journal of pharmacology·2026
Same author

A Pancreatic Ductal Adenocarcinoma Diagnostic System Using Serum Extracellular Vesicle Detection with Optimized Lectin Combination Using Machine Learning.

Cancers·2026
Same author

Mitorubin, berberrubine-based compounds that improve mitochondrial function, exhibit cardioprotective effects against age-related cardiac dysfunction.

npj aging·2026
Same author

Real-Time Detection of Cardiotoxic Anticancer Drug by Diamond Microsensors in the Skin and Combined Monitoring of Electrocardiogram in Live Rats.

ACS sensors·2026

相关实验视频

Updated: Jan 16, 2026

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

1.3K

用基于活动的蛋白质标记探针开发单分子酶活性测试,用于血清水解酶.

Seiya Ishii1, Mayano Minoda1, Tadahaya Mizuno1

  • 1Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, 113-0033, Japan.

Small methods
|September 30, 2025
PubMed
概括

新的单分子测定方法使用基于活动的探测器检测超级家族中的活性酶. 这种方法可以识别血液中的活跃B粒酶,这可能表明肝损伤和免疫激活.

关键词:
化学生物学 化学生物学酶是一种酶.酶组学是指酶组学.一个分子分析分析.

更多相关视频

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
09:45

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

10.1K
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

13.2K

相关实验视频

Last Updated: Jan 16, 2026

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

1.3K
Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
09:45

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

10.1K
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

13.2K

科学领域:

  • 生物化学 生化学
  • 分子生物学分子生物学
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • 单分子酶活性测定提供了对蛋白质形式水平上异常蛋白质功能的洞察.
  • 一个关键的限制是当前测定方法可以准的酶数量有限.

研究的目的:

  • 开发新的单分子酶活性测试,能够针对更广泛的酶.
  • 为了利用基于活动的探针来对活性酶进行超级家族范围的标签.

主要方法:

  • 开发单分子酶活性测试,使用基于活性的探针.
  • 使用基于酸的探针进行的原则证明研究.
  • 在血液中的单分子水平检测活性血清酸酶,包括PSA和B粒酶.

主要成果:

  • 在血液等复杂的生物样本中,证明了在单分子水平上检测活性氨酸酸酶的能力.
  • 在血液样本中成功识别了活性B粒酶.

结论:

  • 血液中活跃的B粒酶显示出作为肝损伤生物标志物的潜力.
  • 这种生物标志物与免疫细胞激活有关,这表明肝损伤和免疫反应之间存在联系.