频谱静音和光学透明:Clear-SiR用于深度拉曼生物分子传感
Michael Ka Ho Lee1, Kenta Mizushima2, Peng Zheng1
1Department of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States.
ACS sensors
|September 30, 2025
概括
在静音区域 (Clear-SiR) 中用于分析拉曼的清除启用光增强通过减少光散射和光谱干扰来改善深层组织成像. 这种新的方法增强了拉曼光谱检测深度,用于在生物组织中准确的生化传感.
科学领域:
- 生物光子学和光谱学
- 医学成像和诊断 医学成像和诊断
背景情况:
- 拉曼光谱 (RS) 提供无标签的分子识别,但在深层组织成像中受到光散射和内源光谱干扰的限制.
- 使用振动标签的拉曼静音区域 (1800-2800 cm-1) 可以最大限度地降低背景噪声,但由于折射率 (RI) 不匹配,组织光学不透明度会减弱信号.
- 克服这些光谱和光学障碍对于深层组织生化传感至关重要.
研究的目的:
- 通过生物组织引入Clearing-enabled Light Enhancement for Analytical Raman in the Silent Region (Clear-SiR),这是一种通过生物组织进行高准确度拉曼检测的混合方法.
- 通过组织清除将折射率调制与在拉曼静音区域的光谱准相结合.
- 为了增强光学透明度,减少光散射,使光线更深入地透.
主要方法:
- 清晰SiR采用FDA批准的染料tartrazine,以同质化组织RI,增强基于克莱默斯-克罗尼格关系的透明度.
- 该方法针对使用4-aminobenzonitrile (4-ABN) 作为记者分子的拉曼静音区域.
- 开发了一种基于凝的快速tartrazine输送协议,用于临床和临床应用.
主要成果:
- 清晰SiR在红色 (638 nm) 和近红外 (785 nm) 激发下显示的探测深度增加了近两倍.
- 该技术成功地克服了生物组织中的光谱干扰和光学散射限制.
- 高准确度,通过皮肤进行拉曼检测是通过最小的内源信号分心来实现的.
结论:
- 通过将物理组织清除与光谱向相结合,Clear-SiR建立了一个强大的平台,以在临床相关的深度探测分子架构.
- 该方法能够准确,深层组织生化传感,为非侵入性分子表型化铺平了道路.
- Clear-SiR支持对有机体和完整组织中的病理生理过程进行纵向监测.
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