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多种药物排泄和Mucor lusitanicus的天生的醇耐药性

Stephanie Toepfer¹, Erwin Lamping², Jasper E James¹

¹Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.

The Journal of antimicrobial chemotherapy

|September 30, 2025

概括

Pleotropic药物耐药性 (PDR) 载体 Pdr1 和 Pdr6 是 Mucor lusitanicus 醇耐药性的关键. 这项研究确定了它们在赋予各种醇抗真菌剂耐药性的作用.

科学领域:

菌类学菌类学是指菌类学.
分子生物学分子生物学
抗微生物耐药性抗微生物耐药性

背景情况:

卢西塔尼克斯真菌是一种新兴的真菌病原体.
醇抗真菌药物对于治疗侵袭性真菌感染至关重要.
了解Mucor物种的耐药性机制对于有效治疗至关重要.

研究的目的:

为了研究八种类药物耐药性 (PDR) 载体在Mucor lusitanicus中醇耐药性中的作用.
描述M. lusitanicus PDR载体的功能和表达模式.

主要方法:

基因表达分析使用RNA测序和RT-qPCR对暴露于醇的M. lusitanicus进行.
在Saccharomyces cerevisiae中过度表达GFP标记的M. lusitanicus PDR载体.
评估工程酵母菌株的醇敏感性和血ATPase活性.

主要成果:

卢西塔尼克斯 PDR 载体的基因组分为A (pdr1,pdr6-8) 和B (pdr2-5).
暴露于沃里可纳,伊萨夫可纳和波萨可纳导致pdr1和pdr6的显著上调,pdr7和pdr8的下调.
Pdr1和Pdr8赋予了泛醇耐药性,而Pdr6和Pdr7则赋予了对特定醇类型的耐药性. Pdr3和Pdr5没有显示出排水的功能.

结论:

所有的Mucor集群A PDR传送器都充当多药物排放.
Pdr1和Pdr6被确定为Mucor lusitanicus内在醇耐药性的主要贡献者.

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