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鉴定HSV-2 UL13蛋白激酶的病毒激活剂

Naoto Koyanagi^1,2,3, Kosuke Takeshima¹, Saori Shio¹

¹Division of Molecular Virology, Department of Microbiology and Immunology, the Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.

Journal of virology

|September 30, 2025

概括

简单疹病毒2 (HSV-2) UL13蛋白激酶需要病毒辅因子UL55和Us10才能完全活动. UL55是主要激活剂,而Us10是辅助剂,影响HSV-2复制和传播.

科学领域:

病毒学病毒学
分子生物学分子生物学
生物化学生物化学

背景情况:

疹病毒编码的保护蛋白激酶 (CHPKs) 调节细胞过程.
阿尔法疹病毒CHPKs与其他疹病毒家族的不同,需要病毒辅因子才能完全活动.
简单疹病毒2 (HSV-2) UL13蛋白激酶在受感染细胞中的功能尚未完全理解.

研究的目的:

调查HSV-2 UL13激酶活性对病毒辅因子的要求.
为了识别激活HSV-2 UL13的特定病毒蛋白.
阐明UL13及其激活剂在HSV-2复制和细胞传播中的作用.

主要方法:

在哺乳动物细胞中,UL13与潜在的辅因子 (UL55,Us10) 的同时表达.
在体外激酶试验测量UL13活性.
共同沉测试用于评估蛋白质相互作用.
在UL55和Us10中生成HSV-2无突变.
在感染细胞中分析病毒复制和细胞传播.

主要成果:

UL13与UL55或Us10的同时表达显著增强了EF-1δ酸化.
UL13与UL55和Us10直接相互作用,增加了其激酶活性.
UL55-null突变显著降低了EF-1δ酸化和HSV-2复制/传播.
Us10-null突变的作用很小,但双-null突变进一步降低了酸化.
UL13激酶活性在病毒复制中的作用在很大程度上取决于UL55.

结论:

HSV-2 UL55作为UL13激酶活性的主要激活剂.
HSV-2 Us10 作为 UL13 的辅助激活器.
UL55对于UL13介导的酸化,病毒复制和细胞传播至关重要.
这项研究揭示了阿尔法疹病毒CHPK调节和辅因子依赖激活的新机制.

关键词:

在HSV-2病毒中,UL13 蛋白质激酶蛋白质激酶激活器激活器是什么

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