解读Lolium multiflorum中硫甲基的双抗路径:目标站点和非目标站点洞察
Pei Zhang1,2, Xinyang Yuan3,4, Yuqing Liu3,4
1School of Plant Protection and Environment, Henan Institute of Science and Technology, Xinxiang, 453003, China. zhangpei0519@hist.edu.cn.
Plant cell reports
|September 30, 2025
概括
在中国的Lolium multiflorum已经对中硫甲基除草剂产生了耐药性. 这种耐药性是由于ALS基因的突变,在一些人群中,增强的代谢途径涉及细胞染色体P450.
科学领域:
- 农业科学 农业科学
- 植物生物学 植物生物学
- 生物化学 生物化学
背景情况:
- 洛多花是一种在小麦田中的竞争性杂草,导致大量的产量损失.
- 杂草中的除草剂耐药性是全球日益关注的农业可持续性的问题.
- 梅索硫甲基是一种广泛使用的除草剂,用于控制L. multiflorum等草杂草.
研究的目的:
- 研究来自中国河南省的Lolium多花种群中中硫甲基耐药性的机制.
- 为了确定特定的遗传突变和代谢途径赋予除草剂耐药性.
- 探索对抗除草剂耐药性L. multiflorum.管理的潜在策略.
主要方法:
- 剂量反应测试用于确定不同人群中的耐药性水平.
- 对ALS基因的基因测序以确定目标位突变.
- 使用P450抑制剂 (马拉,PBO) 来评估代谢抵抗的酶抑制测定.
- 分子对接模拟以评估突变对除草剂结合的影响.
主要成果:
- 两种L.多种花种群 (HZ1和HZ2) 显示出对甲硫的显著耐药性 (分别为12.38倍和24.19倍).
- 在两种耐药群体中都发现了ALS基因 (Pro-197-Thr和Asp-376-Glu) 的新突变.
- HZ1的耐药性仅仅是由于位突变,而HZ2则表现出多重耐药性,涉及位突变和增强的P450代谢.
- HZ2中的耐药性被P450抑制剂部分逆转,证实了代谢解毒的作用.
- 抗药性种群对异原的敏感性增加,表明交叉耐药性模式.
结论:
- 这项研究提供了第一个证据,证明中国L. multiflorum中对硫甲基的目标部位和非目标部位的同时耐药性.
- 即使在地理上接近的种群中,也存在不同的耐药机制,这凸显了除草剂耐药性进化的复杂性.
- 了解这些机制对于开发有效的耐药性管理策略至关重要,包括使用替代除草剂,如isoproturon.
相关概念视频
Conservative Site-specific Recombination and Phase Variation
6.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.7K
Responses to Salt Stress
14.5K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.5K
Cell Signaling in Plants
6.1K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.1K
Adaptations that Reduce Water Loss
27.9K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
27.9K
Defenses Against Pathogens and Herbivores
29.5K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
29.5K
Ligand Binding and Linkage
5.5K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
5.5K


