在禽病原性大肠杆菌中扩展谱β-乳糖酶的发生率和评估
Anupriya1, Kavitha Guladahalli Manjunatha1, Abhirami Somasekharan1
1Nitte (Deemed to be University), Nitte University Centre for Science Education and Research (NUCSER), Mangalore, India.
概括
扩展谱β-乳糖酶 (ESBL) 产生禽病原菌大肠杆菌 (APEC) 在家禽养殖场中很普遍. 这项研究发现了高抗生素耐药性,并在废物样本中确定了ESBL生产的APEC,突出了公共卫生问题.
科学领域:
- 兽医微生物学 兽医微生物学
- 抗微生物耐药性 抗微生物耐药性
- 食品安全 食品安全
背景情况:
- 产生禽病原菌大肠杆菌 (APEC) 的扩展谱β-乳糖酶 (ESBL) 是家禽生产中的重大威胁.
- 生物膜形成,毒性因素和耐药性有助于APEC的生存和传播.
- 养场是ESBL生产APEC的潜在储库,对动物和人类健康构成风险.
研究的目的:
- 在家禽养殖场废物样本中调查ESBL产生的APEC的流行情况.
- 确定抗生素耐药性模式,并确定大肠杆菌分离物中的毒性基因.
- 评估产生ESBL的APEC菌株的存在及其遗传特征.
主要方法:
- 从10个家禽养殖场收集了30个废弃物样本.
- 使用埃氨基甲蓝来分离大肠杆菌.
- 使用聚合酶链反应查抗生素耐药性和APEC毒性基因.
- 对ESBL生产的表型确认.
主要成果:
- 所有28个大肠杆菌分离体都对至少一种抗生素表现出耐药性.
- 38%的分离物被确定为APEC菌株.
- 最常见的ESBL基因组合是blaSHV和blaTEM.
- 一个隔离物被表型证实产生ESBL.
结论:
- 高水平的抗生素耐药性和ESBL生产的APEC在家禽养殖场废物中的存在令人担忧.
- 有效的预防措施对于减少ESBL产生的APEC流行和传播至关重要.
- 需要进一步的研究来控制家禽中抗菌素耐药性的传播.
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