血回收驱动在Toxoplasma gondii细胞内复制过程中形成水库
Julia von Knoerzer-Suckow1, Eva-Helena Aden1, Romuald Haase2
1Chair of Experimental Parasitology, Ludwig-Maximilians-University Munich, Planegg-Martinsried, Germany.
PLoS biology
|September 30, 2025
概括
在复制过程中,Toxoplasma gondii寄生虫通过内细胞和外细胞再循环宿主血. 这一过程涉及膜储存器,对于维持膜组织和寄生虫发育至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 寄生虫学的寄生虫学
- 膜贩卖 膜贩卖 膜贩卖
背景情况:
- 像Toxoplasma gondii这样的阿皮复合体寄生虫,居住在宿主衍生的真空中,但可以通过微孔通过内细胞化将宿主蛋白内化.
- 微孔的稳定性与域蛋白K13有关,也与Plasmodium falciparum中的素抵抗有关.
- K13的枯竭表明了微孔在宿主血 (PM) 恒温的作用.
研究的目的:
- 在Toxoplasma gondii的细胞内发育过程中描述宿主血膜 (PM) 的动态和循环.
- 调查内细胞和外细胞在PM恒温和寄生虫复制中的作用.
- 为了确定关键的分子参与者参与PM动态在寄生虫复制过程中.
主要方法:
- 在细胞内的PM动态的特征 Toxoplasma gondii.
- 研究Rab5b和MyoF在寄生虫内外细胞循环中的作用.
- 对扰乱内外细胞平衡对PM组织和储库形成的影响的分析.
主要成果:
- 在细胞内复制过程中,Toxoplasma gondii表现出PM内和外的循环,依赖于Rab5b和MyoF.
- Rab5b对于T. gondii的溶液周期是不可或缺的,与Plasmodium falciparum不同.
- 寄生虫在复制过程中形成细胞外等离子膜储存库 (PMR);破坏内外细胞平衡导致异常的PMR形成和膜失调.
结论:
- 内外细胞循环对于调节宿主血动态和在Toxoplasma gondii复制过程中形成血水库至关重要.
- 这一循环的破坏严重损害了膜组织,突出显示了内细胞和PM恒温之间的联系.
- Rab5b在PM回收过程中发挥作用,但对T. gondii的溶液循环并不必不可少.
更多相关视频
11:21Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
Published on: March 12, 2015
11.4K
11:37QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
Published on: June 22, 2017
16.8K
相关概念视频
00:58Recycling Endosomes and Transcytosis
3.5K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
3.5K
01:22Enlargement of the Plasma Membrane
2.2K
Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
2.2K
01:24What are Membranes?
18.3K
A cell's plasma membrane demarcates the cell's borders and determines the nature of its interaction with the environment. Cells exclude certain substances, take in others, and excrete some others in controlled quantities. The plasma membrane must be flexible to allow certain cells, such as red and white blood cells, to change their shape while passing through narrow capillaries. These are the more obvious plasma membrane functions. In addition, the plasma membrane's surface carries...
18.3K
01:27Diversity of Protists II
786
Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...
786
01:19Membrane Asymmetry Regulating Transporters
6.9K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
6.9K
01:26Membrane Fluidity
14.5K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
14.5K