基于L-RNA Aptamer的工具用于G-四重复结构:识别,表征和应用
Danyang Ji1, Kun Zhang2, Maryana Yarshova2
1State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China.
Accounts of chemical research
|September 30, 2025
概括
在生物系统中,L-RNA体为准G-四重复结构提供了增强的稳定性. 这些新型的体对开发G-四重复相关疾病的诊断和治疗方法充满希望.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 化学生物学 化学生物学
背景情况:
- 亚体,DNA或RNA寡核酸,在生物环境中面临稳定性问题.
- 使用非自然核酸的L-RNA体,提供了对核酶的增强稳定性.
- L-RNA 亚体使得基于结构的选择成为可能,这对于准G-四重复 (G4) 结构至关重要.
研究的目的:
- 开发和应用L-RNA体来准功能G-四重复 (G4) 结构.
- 为了提高对G4位的aptamer选择的效率,结合亲和力和特异性.
- 在各种应用中探索SELEX后的修改,以改善L-RNA吸收酶的功能.
主要方法:
- 开发新的L-RNA胺体选择平台.
- 通过指数式丰富 (SELEX) 增强联体的系统进化,用于G4结构.
- 体的生物物理和生物化学表征.
- 后SELEX修改包括循环化和结合.
主要成果:
- 建立了针对G4结构的强大的L-RNA胺体选择平台.
- 证明提高了SELEX的效率,结合亲和力和特异性.
- 为体外,细胞内和体内应用开发了改性L-RNA体 (圆形,合物).
- 展示了L-RNA体调节G4介导细胞过程的能力.
结论:
- 对于可访问性,效率和稳定性而言,L-RNA 体技术是先进的.
- 对于与G4失调相关的疾病的诊断和治疗,L-RNA体具有显著的潜力.
- 目前正在进行的研究旨在完善L-RNA体的选择,表征,修饰和应用.
相关概念视频
01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
01:56Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
02:35RACE - Rapid Amplification of cDNA Ends
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
Since the...