血清素生物合成酶T5H5和转录因子bZIP23调节了果中光介导的安东素积累
Lingling Lv1, Lei Bi1, Huiru Zhang1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.
暴露于光线会通过通过MdT5H5.5.5.增加血清素 (5-基三胺,5-HT) 通过MdT5H5.5.增加产生的产量而减少果的颜色. 一种抑制剂,MdbZIP23,通过抑制MdT5H5.5.的作用促进了安东的积累.
科学领域:
- 植物生理学 植物生理学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 血清素 (5-基三胺,5-HT) 具有不同的植物功能.
- 安西是水果颜色中的关键颜料.
- 血清素在光诱导的安托西亚宁积累中的作用尚不清楚.
研究的目的:
- 研究5-HT合成酶MdT5H5在果果中因光诱导的安托西亚宁积累中的功能.
- 阐明连接血清素和安托氨酸生物合成的分子机制.
主要方法:
- 对MdT5H5对光反应的基因表达分析.
- 在果果和果实中,MdT5H5的过度表达和沉默.
- 识别调节MdT5H5.5.的转录因子.
主要成果:
- 暴露于光线增加了果果中的MdT5H5表达和血清素水平.
- 过度表达MdT5H5减少了安托西亚宁积累,而沉默增加了它.
- MdbZIP23被确定为MdT5H5的抑制剂,促进了氨酸的积累.
结论:
- MdT5H5通过产生血清素来负面调节果果中因光诱导的安托西亚宁积累.
- MdbZIP23通过抑制MdT5H5.5.的作用促进了酸的积累.
- 这项研究揭示了一种新的分子途径,用于光诱导的水果颜色.
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