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LaACS的计算指导蛋白质工程与SAM合成酶选相结合,协同促进了E中的ACC生产. 大肠杆菌

Yupeng Wan^1,2,3,4, Long Jiang^1,2,3,4, Yaojie Liu^1,2,3

¹CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.

Journal of agricultural and food chemistry

|September 30, 2025

概括

工程细菌现在生产1-aminocyclopropane-1-carboxylic acid (ACC),一种植物激素前体,其产量是以前的两倍. 这一突破提高了用于农业和医药应用的ACC生产.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
植物科学植物科学

背景情况:

1-氨基cyclopropane-1-carboxylic 酸 (ACC) 是一个重要的植物激素前体,调节生长和抗压能力.
提高ACC产量对于农业和潜在的药物应用至关重要.

研究的目的:

开发一种有效的生物合成过程,用于大肠杆菌中的ACC.
通过酶工程和代谢途径优化来提高ACC产量.

主要方法:

使用来自黄金的1-aminocyclopropane-1-carboxylate synthase (LaACS) 进行工程化*大肠杆菌*.
蛋白质结构预测 (AlphaFold2) 和分子对接 (MOE) 确定了一个高活性的LaACS突变体 (LaACS^L243A).
选了*S*-adenosylmethionine合成酶 (SAMS) 和优化了相关的氨酸通路基因 (MetA,MetH).

主要成果:

LaACS^L243A突变显示了显著改善的催化效率 (Vmax翻了一番,增加了Kcat和Kcat/Km).
来自Arabidopsis thaliana的SAMS被确定为最活跃的SAMS.
大肠杆菌中SAMSa,MetA和MetH基因的过度表达使ACC产量增加到109 mg/L,使未经改性菌株的产量翻了一番.

结论:

在大肠杆菌中开发了一种绿色,安全和高效的ACC生物合成过程.
酶工程和代谢途径优化是提高ACC产生的有效策略.
改进的工艺为ACC在农业,生物学和医学中的应用提供了显著的潜力.

关键词:

1-氨基cyclopropane-1-糖酸合成酶的使用情况.生物合成生物合成生物转化过程中的生物转化.分子对接的分子对接.位点定向突变是指向位点的突变.

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