在斑马鱼中对 perfluorooctanoic 酸毒性的聚烯调制:转录和毒理学见解
Yuanyuan Zhao1, Siyi Zhang2, Yu Liu2
1MOE Key Laboratory of Resources and Environmental Systems Optimization, College of Environmental Science and Engineering, North China Electric Power University, Beijing 102206, China; Key Laboratory of Groundwater Resources and Environment, Ministry of Education, College of New Energy and Environment, Jilin University, Changchun 130012, China.
Journal of hazardous materials
|September 30, 2025
概括
聚乙烯微塑料通过改变PFOA生物积累和破坏关键生物通路,加剧斑马鱼中 perfluorooctanoic acid (PFOA) 的毒性. 这种相互作用凸显了水域污染物同时出现的风险.
科学领域:
- 环境毒理学环境毒理学
- 水生生态毒理学 水生生态毒理学
- 分子毒理学 分子毒理学
背景情况:
- perfluorooctanoic 酸 (PFOA) 和微塑料是普遍存在的水污染物.
- FOA和微塑料的组合毒理效应尚未得到充分理解.
- 斑马鱼模型对于评估水生污染物影响至关重要.
研究的目的:
- 调查聚钢 (PS) 微塑料对斑马鱼PFOA毒性的影响.
- 为了阐明PS微塑料对PFOA毒性的尺寸相关影响.
- 探索PFOA-PS相互作用的转录学,毒理学和分子动力学.
主要方法:
- 斑马鱼单独暴露在PFOA和不同大小的PS微塑料 (0.2,2,20微米) 中.
- 转录组分析以确定受影响的生物途径.
- 毒理学评估,包括生存率和组织病理学.
- 分子动力学模拟以了解结合相互作用.
主要成果:
- 单独使用PFOA就会破坏神经递质释放通路.
- 微塑料PS调节PFOA毒性以大小依赖的方式.
- 0.2微米PS增强了PFOA的神经毒性; 2微米PS加剧了氨基酸代谢干扰.
- PS增加了PFOA的积累,降低了生存率,并恶化了PFOA引起的器官损伤.
- 分子模拟显示PS改变蛋白相互作用,影响新陈代谢和排毒.
- 20微米PS放大PFOA抑制第二阶段结合通路.
结论:
- 聚乙烯微塑料显著改变了斑马鱼的PFOA生物积累和毒性.
- 微塑料的尺寸在调节PFOA的毒理结果方面发挥着至关重要的作用.
- 微塑料和PFOA的同时存在给水生生态系统带来了相当大的环境风险.
相关概念视频
02:04Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
01:27Effects of Chemicals: Overview
Drugs, encompassing various chemical compounds from natural sources, lab synthesis, or genetic engineering, elicit different biological responses in living organisms. Some of these responses are desirable or therapeutic, while others are undesirable. The primary goal of administering a drug is to achieve a therapeutic effect, that is, to address a specific disease or health condition. Any concurrent effects outside of this therapeutic outcome are considered undesirable. These undesirable...
01:36Types of Toxins
Humans continually engage with an environment rich in potentially harmful chemicals. These are introduced to our bodies through inhalation, ingestion, or skin contact. These chemicals exist in various forms, such as air and environmental pollutants, agricultural chemicals, organic solvents, and heavy metals.
Air pollutants, primarily gases, pose significant threats to respiratory health, leading to conditions like hypoxia, lung cancer, and in extreme cases, death.
Environmental pollutants like...
Air pollutants, primarily gases, pose significant threats to respiratory health, leading to conditions like hypoxia, lung cancer, and in extreme cases, death.
Environmental pollutants like...
01:23Toxicity Testing in Animals
Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...
01:28Gene Regulation in Microbial Communities: Quorum Sensing
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
01:28Regulation of Bacterial Virulence
Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...