在S100A11/ANXA2轴中介于电传导以促进工程心肌组织中电气诱导的收缩
Li Xue1, Qi Zhang2, Yijing Wang1
1State Key Laboratory for Manufacturing Systems Engineering, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Center for Mitochondrial Biology and Medicine, School of Life Science and Technology, International Joint Laboratory for Micro/Nano Manufacturing and Measurement Technology, Xi'an Key Laboratory for Biomedical Testing and High-end Equipment, Xi'an Jiaotong University, Xi'an, 710049, Shaanxi, PR China.
研究人员确定了S100A11/ANXA2蛋白质复合体作为心脏组织工程中的关键调解器. 电刺激可以提高这种复杂的调节,增强心肌细胞功能,促进组织成熟,从而为潜在的心脏修复疗法提供帮助.
科学领域:
- 生物医学工程 生物医学工程
- 心血管研究研究心血管研究
- 分子生物学分子生物学
背景情况:
- 缺血性心脏病 (IHD) 由于电气完整性受到破坏,导致心脏功能受损.
- 工程功能性心肌组织需要理解细胞外电微环境调制.
- 工程心脏组织成熟的机制尚未完全理解.
研究的目的:
- 确定工程心脏组织中电生理功能和成熟的关键介质.
- 阐明电刺激在心脏组织发育中的作用.
- 探索心脏再生的治疗目标.
主要方法:
- 使用一个电气工程平台,带有异性减少的氧化石墨烯/丝纤维素 (rGO/Silk) 脚手架.
- 应用电刺激 (ES) 到新生小鼠心肌细胞 (NRCMs).
- 研究了蛋白质表达,复合体形成,动态和基因转录.
主要成果:
- 鉴定了S100结合蛋白A11 (S100A11) 作为一个关键的电响应介质,与Annexin A2 (ANXA2) 形成复合体.
- 电刺激上调了S100A11/ANXA2,增强了NRCM的电生理功能 (流入,膜潜力,CICR).
- 电线索诱导了S100A11的核转移,上调了Gata4和Zfpm1,从而促进了细胞骨蛋白 (F-actin,α-actinin,β-Tubulin) 改善了体组织和E-C合.
结论:
- S100A11/ANXA2轴是心肌组织成熟过程中的关键电-生物介质.
- 这个轴将电调节与增强的心肌细胞功能和细胞骨重塑联系起来.
- 这些发现对心脏再生策略有治疗意义.
相关概念视频
Smooth Muscle Contraction
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
Electrophysiology of Normal Cardiac Rhythm
Specialized Characteristics of Cardiac Muscles
Cardiac muscle cells are smaller than skeletal muscles, averaging 10–20 mm in diameter and 50–100 mm in length. However, they have large energy demands for continuous contraction and relaxation. This energy is almost exclusively derived from aerobic metabolism of energy...
Excitation-Contraction Coupling in Skeletal Muscles
When an action...
Conduction System of the Heart
The pacemaker cells are located in two primary nodes: the sinoatrial (SA) node and the atrioventricular (AV) node. The SA node pacemaker cells can autonomously depolarize, triggering an action potential that leads to the...


