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在精子微注射过程中使用两种介质的辅助生殖技术结果之间的比较:二碳酸盐介质 (单步) 与HEPES缓冲介质 (SynVitro Flush) 相比

Akram Hosseini^1,2, Zahra Borzouei^1,2, Elham Naghshineh^2,3

¹Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Clinical and experimental reproductive medicine

|September 30, 2025

概括

与HEPES缓冲介质相比,二碳酸介质显著改善了细胞内体精子注射 (ICSI) 结果,导致更高的受精,胚胎质量和活产率. 这凸显了介质选择在辅助生殖技术 (ART) 中的关键作用.

科学领域:

生殖生物学生殖生物学
细胞培养媒体细胞培养媒体
辅助生殖技术 (ART) 是一种辅助生殖技术.

背景情况:

在ART中,基乙基烯酸乙硫尼酸 (HEPES) 缓冲介质是常见的,但它们对细胞机制的影响仍在争论中.
在细胞内精子注射 (ICSI) 期间,对二碳酸盐与HEPES缓冲介质进行了有限的比较研究.

研究的目的:

在ICSI程序中,比较碳酸介质 (单步) 与HEPES缓冲介质 (SynVitro Flush) 的疗效.
评估不同媒体对关键ART结果的影响.

主要方法:

从2023年开始对200个ICSI周期进行了回顾性分析.
在ICSI过程中将第二阶段卵细胞分配到二碳酸盐 (SAGE 1-STEP) 或HEPES缓冲媒体 (SynVitro Flush) 中.
两组之间的受精,胚胎发育,植入,怀孕和活产率的比较.

主要成果:

与SynVitro介质 (82.30%;p<0.001) 相比,单步介质的受精率 (89.04%) 显著更高.
改善了胚胎质量 (更多的胚胎分裂,更高的A / B类胚芽细胞) 和增加了胚胎冷在单步组.
在单步组中显著更高的临床怀孕和活产率 (p<0.05).

结论:

一级碳酸介质提高了ICSI循环效率,而不是SynVitro Flush HEPES缓冲介质.
适当的介质选择对于优化ART结果至关重要.
媒体之间没有观察到流产或多胎妊娠率的显著差异.

关键词:

双碳酸的介质是二碳酸.在冷的胚胎.在HEPES的缓冲中,HEPES的缓冲器人类胚胎发育的发展.人类胚胎质量的人类胚胎质量.怀孕率怀孕率怀孕率精子注射,细胞体内注射.

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