HSP90在等离子体膜上差异稳定植物ABCB类型的辅酶载体
Tashi Tsering1, Martin Di Donato1, Despina Samakovli2
1University of Fribourg, Department of Biology, Fribourg, Switzerland.
植物FKBP42/TWISTED DWARF1 (TWD1) 蛋白稳定了植物ATP结合盒 (ABC) 载体,这对辅酶的运输和发育至关重要. 这项研究揭示了HEAT-SHOCK PROTEIN90 (HSP90) 通过TWD1.0动态调节这些ABCB载体.
科学领域:
- 植物分子生物学 植物分子生物学
- 细胞运输机制的细胞运输机制.
- 蛋白质与蛋白质的相互作用
背景情况:
- FKBP42/TWISTED DWARF1 (TWD1) 和FKBP38分别调节植物和哺乳动物的ATP结合盒 (ABC) 载体.
- FKBP-ABCB传送器相互作用的精确机制在很大程度上仍未被探索.
研究的目的:
- 研究细胞质热冲击蛋白90 (HSP90) 异型在TWD1-ABCB相互作用中的作用.
- 阐明HSP90在调节植物ABCB传送器中的功能机制细节.
主要方法:
- 酵母双杂交查以确定与TWD1.1相互作用的HSP90异型.
- 共同免疫沉和西部涂抹以确认蛋白质相互作用.
- 在植物中药理上抑制HSP90和基因分析.
主要成果:
- 细胞质HSP90异型被确定为TWD1的相互作用体,其结合被映射到TPR域附近的特定α螺旋.
- 与TWD1相互作用的植物ABCB传送器作为构成性HSP90客户端,与它们的哺乳动物对应物不同.
- 抑制HSP90显著影响ABCB血稳定性和循环速率,影响植物发育.
结论:
- TWD1调解HSP90和植物ABCB传送器之间的特定相互作用.
- HSP90在稳定等离子体膜ABCB异型体中发挥着动态作用,调节极极auxin运输.
- 这种调节机制赋予了植物的发育可塑性.
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