CCAAT/增强剂结合蛋白β (C/EBPβ) 调节卵巢储备的形成
Xiaoyuan Zhang1,2, Yue Zeng1, Zhanzhong Qiao1
1College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Communications biology
|October 1, 2025
概括
CCAAT/增强剂结合蛋白β (C/EBPβ) 对于小鼠的卵巢储备形成至关重要. 这种转录因子通过调节基因表达和信号通路来促进原始卵泡组合,这对于卵子发育至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 分子内分泌学分子内分泌学
背景情况:
- 原始卵泡 (PF) 池或卵巢储备是在胚胎发育期间建立的,并且是有限的.
- 转录因子CCAAT/增强剂结合蛋白β (C/EBPβ) 在成年卵巢中存在,但其在卵巢早期发育中的作用尚不清楚.
研究的目的:
- 研究C/EBPβ在小鼠早期卵巢发育期间形成原始卵泡池中的作用.
- 阐明C/EBPβ调节原始毛囊组合的分子机制.
主要方法:
- 在小鼠原始毛囊形成期间分析C/EBPβ表达和局部化.
- 在卵巢细胞中C/EBPβ的体外淘汰.
- 染色体可访问性分析 (ATAC-seq).
- 对基因表达和信号通路激活的评估.
主要成果:
- 在小鼠原始卵泡形成过程中,C/EBPβ表达得到增强,并在原始卵泡组装过程中转移到细胞核.
- 降低C/EBPβ抑制了关键蛋白质的表达,并阻碍了原始卵泡的形成.
- 通过与其促进体结合,C/EBPβ促进了Ep300的表达,并维持了Furin促进体的染色体可访问性,增强了神经色素受体激酶 (NTRK) 信号传递.
- 核转移C/EBPβ受酸化和Lamin B1.1的调节.
结论:
- 在小鼠中,C/EBPβ是建立卵巢储备的关键调节剂.
- 通过对基因的表观遗传调节和对重要信号通路的调节,C/EBPβ在原始卵泡组装中发挥着关键作用.
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