相关实验视频

Updated: Jan 16, 2026

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Published on: August 20, 2011

通过PCR对转基因棉花植物进行表征

Janani Sree Sivakumar¹, Dhandapani Gurusamy², Selvi Chellamuthu³

¹Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India.

Methods in molecular biology (Clifton, N.J.)

|October 1, 2025

概括

本研究详细介绍了聚合酶链反应 (PCR) 方法来表征转基因棉花,包括逆转录PCR (RT-PCR) 来分析基因表达并确认抗压力转基因整合.

科学领域:

农业生物技术农业生物技术
分子生物学分子生物学
植物科学植物科学

背景情况:

像棉花这样的转基因作物是为了增强对生物和非生物压力的抵抗力而设计的.
鉴定转基因表达的特征对于理解作物改良至关重要.

研究的目的:

通过分子技术提供详细的协议来表征转基因棉花.
在此背景下解释聚合酶链反应 (PCR) 和逆转录PCR (RT-PCR) 的应用.

主要方法:

详细的原料设计,DNA/RNA提取和cDNA合成协议.
主混合物制备,热循环程序和阿加罗斯凝电泳用于PCR产品分析.
用RT-PCR来量化基因表达水平的应用.

主要成果:

在棉花中验证转基因存在和表达的综合方法.
证明PCR和RT-PCR是转基因植物表征的重要工具.
成功放大和分析DNA和RNA目标.

结论:

PCR和RT-PCR是转基因棉花分子表征不可或缺的技术.
这些方法可以评估转基因的整合和功能表达,有助于作物改进战略.
描述的协议有助于对植物的遗传修饰进行可靠的分析.

关键词:

基因表达基因表达基因表达没有NPTII,没有NPTII.它们是初级的.假设的转基因棉花.

更多相关视频

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems

A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems

Published on: March 23, 2015

相关概念视频

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Transgenic Organisms

Transgenic Organisms