相关实验视频

Updated: Jun 12, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
09:46

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

Published on: February 25, 2010

47.1K

在Pichia pastoris中利用自我处理2A来实现多基因表达.

Katharina Ebner1, Martina Geier2

  • 1bisy GmbH, Wuenschendorf, Hofstätten an der Raab, Austria.

Methods in molecular biology (Clifton, N.J.)
|October 1, 2025
PubMed
概括

这项研究引入了一种新的多基斯特龙通路表达策略,使用Komagataella phaffii (以前是Pichia pastoris) 中的自处理2A. 这种方法简化了用于代谢工程和菌株发展的多基因表达.

关键词:
2A类的类.科玛格塔贝拉 (Komagataella phaffii) 是一个有趣的植物.多酶通路是多种酶的通路.这就是Pichia pastoris.通过多基斯特龙式表达.

更多相关视频

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

13.8K
Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies05:30

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Published on: January 23, 2019

14.4K

相关概念视频

Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...