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Updated: Jan 16, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
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Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

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通过使用CRISPR/Cas9来生成Pichia pastoris高吞吐量敲除菌株的高吞吐量生成

Kirill Smirnov1, Lukas Rieder2, Anton Glieder3

  • 1Christian Doppler Laboratory for Innovative Pichia pastoris Host and Vector Systems, Graz University of Technology, Petersgasse, Graz, Austria. k.smirnov@tugraz.at.

Methods in molecular biology (Clifton, N.J.)
|October 1, 2025
PubMed
概括

这项研究提出了一个快速的CRISPR/Cas9管道,用于生成Komagataella phaffii突变菌株. 该方法可以实现高通量基因组工程,具有易于复制的步骤和可用于进一步研究的等离子体消除.

关键词:
这就是CRISPR/Cas9的作用.基因淘汰赛 基因淘汰赛基因组工程是基因组工程.科玛格塔贝拉 (Komagataella phaffii) 是一个有趣的植物.这就是Pichia pastoris.合成生物学 合成生物学

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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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