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Updated: Jan 16, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
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皮奇亚牧师通过高压同质化 (HPH) 破坏细胞

Antoni Casablancas1, Xavier Garcia-Ortega1, Francisco Valero2

  • 1Department of Chemical, Biological and Environmental Engineering, Engineering School, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.

Methods in molecular biology (Clifton, N.J.)
|October 1, 2025
PubMed
概括

高压均质化 (HPH) 有效地破坏细胞的产品回收. 本指南详细介绍了将HPH应用于Komagataella phaffii酵母细胞工厂,以改善下游加工.

关键词:
细胞的破坏 细胞的破坏关键过程参数 (CPP)高压均化高压均化关键绩效指标 (KPI) 是指一个关键的绩效指标.科玛格塔贝拉 (Komagataella phaffii) 是一个有趣的植物.这就是Pichia pastoris.

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Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
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