使用STARmap PLUS,RIBOmap和TEMPOmap在完整的细胞和组织中的转录组规模的mRNA生命周期空间解析的现场分析
Jingyi Ren1,2, Hu Zeng1,2, Jiahao Huang1,2
1Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA.
Nature protocols
|October 1, 2025
概括
这项研究介绍了STARmap PLUS,RIBOmap和TEMPOmap,这是先进的空间奥米克工具,用于in situRNA和蛋白质分析. 这些方法使细胞和组织中基因表达动态的高分辨率跟踪成为可能.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 细胞生物学 细胞生物学
背景情况:
- 控制基因表达对细胞功能至关重要.
- RNA生命周期决定了精确的蛋白质合成.
- 了解空间和时间RNA动态是至关重要的.
研究的目的:
- 详细介绍一项用于in situ空间奥米克分析的综合协议.
- 能够在完整的细胞和组织中对RNA和蛋白质进行高度多重分析.
- 为追踪数千种RNA物种的空间和时间动态提供工具.
主要方法:
- 这是STARmap PLUS,RIBOmap和TEMPOmap的综合协议.
- 基于成像的,高度多重化的现场分析.
- 将RNA转换为DNA片,用于通过共聚焦显微镜进行现场测序.
主要成果:
- 在完整的细胞和组织中追踪数千种RNA物种的空间和时间动态.
- 生成空间分辨的单细胞概况.
- 协议在配备好的实验室中很容易执行.
结论:
- 与现有方法相比,开发的空间奥米克工具包提供了增强的分析能力.
- 能够进行下游分析,如细胞类型分类和细胞周期识别.
- 促进对异质生物系统中RNA动态的更深入的理解.
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