Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Reporter Genes02:11

Reporter Genes

12.9K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
12.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Systemic regeneration medicines for muscular dystrophy: progress and challenges in pro-regenerative drug development.

Cell regeneration (London, England)·2026
Same author

DMD-Null mice exhibit severe muscle weakness, impaired regeneration, and deficient satellite cell function.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Dystrophin-gene mutation location influences severity of electroretinogram defects in mouse models of Duchenne muscular dystrophy.

BMC medicine·2026
Same author

RNA Therapeutics for Duchenne Muscular Dystrophy: Exon Skipping, RNA Editing, and Translational Insights from Genome-Edited Microminipig Models.

International journal of molecular sciences·2026
Same author

A Novel Dysferlin-Binding Kinase CK2α Promotes Plasma Membrane Repair in Dysferlinopathy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Prostaglandin E2 receptor EP2 is indispensable for maintenance of skeletal muscle stem cells.

Stem cells (Dayton, Ohio)·2026

相关实验视频

Updated: Jan 16, 2026

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
07:44

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Published on: July 4, 2018

8.8K

使用记者小鼠进行拼接调制研究.

Katsura Minegishi1, Tsukasa Tominari1, Yoshitsugu Aoki2

  • 1Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan.

Methods in molecular biology (Clifton, N.J.)
|October 1, 2025
PubMed
概括
此摘要是机器生成的。

反感性寡核酸 (ASO) 为杜申肌肉发育不良 (DMD) 提供外型跳转疗法. 一种新型的记者小鼠模型使得ASO在DMD治疗中的有效性能够进行非侵入性,高通量评估.

关键词:
杜申尼肌肉发育不良症是什么在Exon跳过的情况下,Exon跳过.光是一种光效应.在体内成像系统 (IVIS)路西法拉斯就是路西法拉斯.记者鼠标 记者鼠标

更多相关视频

A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning
09:22

A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning

Published on: June 22, 2015

15.0K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.2K

相关实验视频

Last Updated: Jan 16, 2026

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
07:44

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Published on: July 4, 2018

8.8K
A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning
09:22

A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning

Published on: June 22, 2015

15.0K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.2K

科学领域:

  • 生物化学 生物化学
  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学

背景情况:

  • 杜氏肌肉发育不良 (DMD) 是一种遗传性疾病,其特征是肌肉逐渐退化.
  • 使用反感性寡核酸 (ASOs) 的外显子跳转疗法旨在通过纠正DMD基因的读取框架来恢复双蛋白生产.
  • 目前用于评估ASO疗效的方法,如RT-PCR和对活检组织的免疫涂抹,是劳动密集型和低通量.

研究的目的:

  • 开发一种新的,高通量和非侵入性的方法,以评估异子跳转ASO in vivo的疗效.
  • 为了证明记者小鼠对监测ASO诱导的外因子跳转和随后的蛋白质表达的有用性.

主要方法:

  • 开发了经过成功的外子跳转后,被设计成表达光蛋白的记者小鼠.
  • 用于诱导异构基因跳转的反感性寡核酸 (ASO) 的管理.
  • 在记者小鼠中进行光蛋白表达的非侵入性监测,以评估治疗疗效.

主要成果:

  • 由ASOs诱导的外显子跳转导致记者小鼠中的光蛋白的表达.
  • 报告员系统允许实时,非侵入性评估ASO介导的外跳跃.
  • 这种体内方法为评估下一代ASO疗法提供了一个高通量平台.

结论:

  • 记者小鼠提供了一个强大的工具,用于高吞吐量,非侵入性评估突变突变ASO对杜氏肌肉发育不良的疗效.
  • 这种新的方法简化了基于ASO的治疗策略的评估,加速了药物开发.
  • 在体内报告员系统促进ASO序列和修改的高效选,以实现最佳的外显子跳转诱导.