梅拉斯马病理特征的非侵入性评估:两光子显微镜和反射共聚焦显微镜的并排比较
Xiaoli Ning1,2, Jungang Yang2,3, Hongfei Ouyang2,4
1Department of Dermatology, China-Japan Friendship Hospital, Capital Medical University, Beijing, China.
Pigment cell & melanoma research
|October 1, 2025
概括
双光子显微镜 (TPM) 和反射共聚焦显微镜 (RCM) 提供非侵入性体内瘤病理学的评估. 这些先进的成像技术准确地描述了表皮黑色素和黑色素细胞活动,有助于精确的瘤管理.
科学领域:
- 皮肤病学 皮肤病学
- 医疗成像医学成像
- 光学显微镜的使用方法
背景情况:
- 黑色素,一种常见的超色素障碍,提出了诊断上的挑战.
- 非侵入性 in vivo 病理评估工具对于疹管理至关重要.
- 双光子显微镜 (TPM) 和反射共焦显微镜 (RCM) 是先进的成像方式.
研究的目的:
- 为了比较TPM和RCM在体内特征瘤病理学的疗效.
- 用TPM和RCM评估成像清晰度和特定的病理特征.
- 为了将显微镜检测结果与已建立的瘤评估指标相关联.
主要方法:
- 一项涉及130名黑色素瘤患者的横截面研究.
- 使用TPM和RCM进行体内成像.
- 在显微镜数据,黑色素指数 (MI) 和个体类型角度 (ITA) 之间的斯皮尔曼相关性分析.
- 活跃和稳定乳腺瘤阶段之间的特征比较.
主要成果:
- TPM和RCM在检测表皮黑色素增加,皮肤-表皮结点 (DEJ) 激活的黑色素细胞和平的网状脊柱方面表现出实质性的一致性.
- 在可视化细胞内黑色素,悬浮的黑色素细胞和太阳弹性质方面,TPM表现出色.
- 在DEJ中,RCM更好地识别了激活的黑色素细胞,并且在活跃的黑色素瘤中显示了更多的皮肤炎症细胞.
- TPM量化表皮黑色素与MI正相关,与ITA负相关.
结论:
- TPM和RCM协同提供了全面的体内瘤病理学特征.
- TPM量化表皮黑色素,用于疾病严重程度的评估.
- RCM检测炎症细胞,反映疾病活动.
- 这些互补的成像工具有助于精确管理瘤.
更多相关视频
09:37Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition
Published on: August 18, 2022
2.8K
10:35Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
Published on: October 17, 2016
8.3K
相关概念视频
01:16Confocal Fluorescence Microscopy
20.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
20.0K
01:29Two-Dimensional Microscopy in Microbiology
1.1K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.1K