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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers
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在植物中解码特定组织增强剂,使用大规模并行测试和深度学习.

Yaxin Deng 邓雅欣1, Weihua Zhao 赵伟华1, Yixue Xiong 熊逸雪1

  • 1Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.

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概括

研究人员使用大规模并行报告员测试 (MPRA) 在番茄中确定了数千个活性果实增强剂序列. 这些增强剂可以驱动水果特异性基因表达,从而使新合成增强剂的设计成为有针对性的植物基因调节的目标.

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科学领域:

  • 植物生物学 植物生物学
  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 增强剂是控制植物基因表达的关键调节元素.
  • 了解特定组织增强剂是剖析植物发育和反应的关键.
  • 管理植物增强剂的监管规则,特别是对于水果特异性表达,基本上是未知的.

研究的目的:

  • 在番茄果实中识别和描述功能增强剂序列.
  • 探索果实特异性增强剂活性背后的序列决定因素.
  • 为了使合成增强剂的新设计能够在植物中实现向基因表达.

主要方法:

  • 大规模并行报告测试 (MPRA) 用于测试11180个番茄果特异性基因促进体片段的增强剂活性.
  • 深度学习模型被用来剖析果实增强剂的序列决定因素.
  • 合成增强剂的设计是基于学习的监管规则,并对番茄果的特异性进行实验验证.

主要成果:

  • 在番茄中发现了2,436个活性果实增强剂序列.
  • 确定了一组增强剂的子集,在水果和叶子之间表现出差异性的活性,表明了水果特异性的潜力.
  • 成功设计和验证的合成增强剂能够在番茄果实中进行特定向.

结论:

  • 在番茄果实中建立了功能增强剂的综合景观.
  • 该研究提供了对果实增强剂基于序列的监管代码的见解.
  • 这项工作促进了合成增强剂的合理设计,以精确控制植物中的基因表达.