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基于水溶性方素的NIR染色化探头用于HClO/ClO在环境水中的检测和活细胞生物成像
Huifang Li1, Wanying Li1, Yiru Tang1
1Green Pharmaceutical Technology Key Laboratory of Luzhou City, Central Nervous System Product Research and Development Key Laboratory of Sichuan Province, School of Pharmacy, Southwest Medical University, Luzhou, 646000, PR China.
Chemistry (Weinheim an der Bergstrasse, Germany)
|October 1, 2025
概括
一个新的近红外探测器,SO3SQ,能够灵敏地检测水和细胞中的低酸/低化物 (HClO/ClO-). 这种基于方的染料为环境和生物监测提供了快速,选择性和可见的色度反应.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 低酸/低化物 (HClO/ClO-) 是生物系统中至关重要的活性氧物种.
- 对于环境和细胞研究,需要敏感的HClO/ClO-检测方法.
- 现有的探测器可能缺乏水溶性,特定的检测波长或快速响应时间.
研究的目的:
- 开发一种新的近红外 (NIR) 水溶性探头,用于敏感的HClO/ClO-检测.
- 为了评估探测器在环境水样和活细胞中的性能.
- 扩大方形染料在反应性氧物种传感中的应用.
主要方法:
- 一个基于方的化化探针 (SO3SQ) 的合成.
- 探测器属性的评估,包括水溶性,光谱特征,选择性和响应时间.
- 探头用于环境水样中的定量检测.
- 在HepG2细胞中实时光成像HClO/ClO-.
主要成果:
- SO3SQ表现出良好的水溶性和NIR检测波长 (696nm/725nm).
- 探测器表现出高选择性,快速响应 (在几秒内) 和可见的色度变化.
- 在环境水样中实现了HClO/ClO-的定量检测.
- 在HepG2细胞中对外源和内源HClO/ClO-的实时光成像是成功的,细胞毒性最小.
结论:
- 开发的SO3SQ探针对于敏感和选择性检测HClO/ClO-是有效的.
- 这种探测器在环境和生物环境中为实时监测提供了优势.
- 这项研究突出了四角色素染料在设计先进的NIR水溶性ROS探针方面的潜力.
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