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用于脑瘤光谱的带选择性IR PRESS允许对乳酸盐进行可靠的检测

Shun Kishimoto^1,2, Olga Kim³, Jeeva Munasinghe⁴

¹Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

bioRxiv : the preprint server for biology

|October 1, 2025

概括

一种新的磁共振光谱学 (MRS) 方法SPIR-PRESS改善了瘤中的体内乳酸量化. 这种技术有效地抑制脂质信号,增强乳酸和其他关键代谢物的检测,以便更好地分析瘤微环境.

科学领域:

生物医学工程生物医学工程
分子成像学分子成像学
癌症研究癌症研究

背景情况:

乳酸在瘤微环境中至关重要,影响瘤的进展,转移和免疫逃避.
通过磁共振光谱 (MRS) 在体内定量乳酸是具有挑战性的,因为重叠的脂质信号.
使用长回声时间的传统方法抑制了必需的代谢物信号.

研究的目的:

引入和验证一种新的MRS技术SPIR-PRESS,以改善体内乳酸量化.
克服现有的MRS方法在区分乳酸与脂质信号方面的局限性.
为了在瘤微环境中实现全面的代谢物概况.

主要方法:

开发一种具有短回声时间的选择性反转脉冲MRS方法 (SPIR-PRESS).
在幻影实验中进行评估,以评估脂质抑制和代谢物敏感性.
在小鼠质瘤模型中测试体内乳酸和代谢物检测.

主要成果:

在短TE PRESS光谱中,SPIR-PRESS有效地抑制了干扰乳酸检测的脂质信号.
该方法保持了对全代谢物概况的灵敏度,与传统的长TE技术不同.
与长时间的TE PRESS相比,SPIR-PRESS显示乳酸检测灵敏度增加了60%左右.
在体内,SPIR-PRESS在小鼠质瘤模型中检测到乳酸和其他关键代谢物 (胆,肌酸,NAA).

结论:

在瘤中,SPIR-PRESS提供卓越的乳酸量化和全面的代谢物概况.
这种技术解决了基于MRS的瘤分析中脂质信号重叠的挑战.
SPIR-PRESS代表了研究瘤微环境的一个有前途的进步.

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