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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Jan 16, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

最少侵入性DNA介导光稳定用于扩展单分子和超分辨率成像.

Michael Scheckenbach¹, Cindy Close¹, Julian Bauer¹

¹Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 München, Germany.

bioRxiv : the preprint server for biology

|October 1, 2025

概括

此摘要是机器生成的。

我们开发了一种DNA介导的光稳定方法,以防止在显微镜中进行光光漂白. 这种微创技术提高了成像质量,并扩大了超分辨率显微镜的光孔选项.

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Published on: September 29, 2014

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

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Last Updated: Jan 16, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

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科学领域:

生物物理学的生物物理.
显微镜的使用方法
分子成像学分子成像学

背景情况:

光漂白限制了单分子和超分辨率显微镜.
传统的光稳定方法有缺点,如光体选择和生物破坏.

研究的目的:

开发一种使用DNA介导传递的模块化,最少侵入性的光稳定方法.
在显微镜中克服传统光稳定方法的局限性.

主要方法:

利用DNA介导向成像部位传递光稳定剂.
实施了用于持续补充光稳定器的回收计划.
将该方法应用于3D-DNA PAINT细胞成像.

主要成果:

在较低的激发强度下,DNA介导的策略优于基于溶液的方法.
在显著较低的添加剂度下实现了高效的光稳定.
在3D-DNA PAINT细胞成像中证明了更好的局部化率和精度.

结论:

通过DNA介导的光稳定是一种有前途的解决方案,用于高添加剂度被禁止的成像.
模块化允许适应各种成像方案,并扩展合适的光体.
这种方法提高了单分子和超分辨率成像的能力.