脏TRPM3通道通过管球体反和血体积控制来调节血压
Jorge Rojo-Mencia1, Lucía Alonso Carbajo1,2, Marycarmen Arévalo-Martínez1
1Departamento de Bioquímica y Biología Molecular y Fisiología, Universidad de Valladolid, and Unidad de Excelencia, Instituto de Biomedicina y Genética Molecular (IBGM), CSIC, Valladolid, Spain (J.R.-M., L.A.C., M.A.-M., L.B.-S., M.T.P.-G., J.R.L.L., P.C.).
脏中的TRPM3通道通过影响再吸收和管球体反来调节血压. 缺乏TRPM3的小鼠由于功能受损,血压较低.
科学领域:
- 生理学 生理学 生理学
- 心血管研究研究心血管研究
- 脏生理学 脏生理学
背景情况:
- 瞬态受体潜在的 Melastatin 3 (TRPM3) 通道是感觉神经元,大脑,脏和心血管系统中发现的非选择性阴离子通道.
- 它们在中的表达表明它们在调节心血管功能和血压 (BP) 中起着作用.
研究的目的:
- 通过血管和脏机制研究TRPM3通道在血压调节中的作用.
- 分析Trpm3 - 淘汰赛 (KO) 小鼠的血管表型,以了解它们对高血压的贡献.
主要方法:
- 在基底条件下和药理学挑战后,在野生类型和Trpm3-KO小鼠中进行非侵入性BP监测.
- 在和血管组织中评估TRPM3表达,使用免疫光和RNAscope.
- 使用 perfusion 和压力肌肉学评估 ex vivo 和血管反应.
主要成果:
- Trpm3-KO小鼠表现出血压显著降低和抗抗胰岛素II诱导的高血压.
- KO小鼠表现出尿路分泌量增加和血体积减少,表明低血压的源.
- 在Trpm3-KO小鼠中观察到受损的管状球体反,导致 afferent动脉收缩减少和NaCl再吸收.
结论:
- 通过调节功能,TRPM3通道在血压调节中发挥着至关重要的作用.
- 在Trpm3-KO小鼠中,麻木管球体反会破坏NaCl的再吸收,导致低血压和较低的血压.
- 脏TRPM3通道代表了血压管理的潜在治疗点.
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