Myo-MOVES:一种定制的电刺激系统,用于对3D生物工程肌肉进行功能研究
Martín Ruiz-Gutiérrez1, Ainoa Tejedera-Villafranca1,2, Sergi Pujol-Pinto1
1Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), C/Baldiri Reixac 10-12, E08028 Barcelona, Spain. jramon@ibecabrcelona.eu.
Lab on a chip
|October 1, 2025
概括
Myo-MOVES平台为3D骨肌肉组织的电脉冲刺激 (EPS) 提供了一个可扩展和可适应的解决方案. 该系统有效地模拟杜恩肌肉发育不良 (DMD) 现象型,有助于肌肉疾病的药物查和治疗开发.
科学领域:
- 生物医学工程 生物医学工程
- 组织工程是组织工程.
- 肌肉骨研究 研究
背景情况:
- 电脉冲刺激 (EPS) 在体外肌肉收缩研究中至关重要.
- 现有的EPS系统在可扩展性,成本和实验灵活性方面面临限制.
- 需要先进的平台来研究3D骨肌肉组织和相关疾病.
研究的目的:
- 介绍Myo-MOVES平台,一个直观而适应的EPS系统用于3D骨肌肉组织.
- 为了验证Myo-MOVES平台的技术能力和概念验证实用性.
- 通过使用3D骨肌肉模型,研究杜氏肌力发育不良 (DMD) 中的膜损伤.
主要方法:
- 开发了Myo-MOVES平台,配备了与24井板兼容的选择器和刺激器.
- 使用石墨电极为3D骨肌样本传送电信号.
- 在DMD工程肌肉组织中评估了力量生成和膜损伤 (埃文斯蓝色染料吸收,肌酸酶释放).
主要成果:
- Myo-MOVES平台证明了单个或多个井的有效有针对性的刺激.
- 验证了该系统在DMD3D骨肌组织中诱导和研究功能相关疾病表型的能力.
- 在DMD模型中成功检测了收缩诱导的肉膜损伤,并测量了力生成.
结论:
- Myo-MOVES平台是一个可行的多功能工具,用于诱导和研究3D骨肌肉中的疾病表型.
- 该系统在药物查和推进DMD和其他肌肉疾病的研究方面具有重大潜力.
- Myo-MOVES促进了3D骨肌肉组织工程应用,为复杂的研究提供了实用解决方案.
相关概念视频
01:20Motor Unit Stimulation
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When the neuron of a motor unit fires an action potential, it triggers a series of events, leading to a twitch contraction in the muscle fibers. The process of excitation-contraction coupling is crucial in relaying the action potential to the muscle fibers.
The latent period of contraction marks the onset of excitation-contraction coupling, when the action potential propagates across the sarcolemma, preparing the muscle fibers for contraction. As the fibers enter the contraction phase, the...
The latent period of contraction marks the onset of excitation-contraction coupling, when the action potential propagates across the sarcolemma, preparing the muscle fibers for contraction. As the fibers enter the contraction phase, the...
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01:22Muscle Stimulation Frequency
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The contraction strength of muscles is regulated by motor neurons, which modulate the frequency of action potentials dispatched to the motor units based on the body's requirements. This process of varying the muscle stimulation frequency allows muscles to contract with a force that is precisely tailored to the needs of the moment, whether lifting a feather or a heavy box.
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...
Wave summation
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01:24Generation of Action Potential in Skeletal Muscles
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Like neurons, muscle cells are also regarded as excitable due to their capacity to change in response to stimuli, primarily due to voltage-gated ion channels embedded in their plasma membranes, which get activated by alterations in the...
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