相关实验视频

Updated: Jan 6, 2026

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

核细胞组的动态再分配和核细胞组灵敏度的增加是THP-1巨对LPS的反应的基础

Jane M Benoit¹, Brandon D Buck¹, Mahdi Khadem¹

¹Department of Biological Science, Florida State University, 319 Stadium Drive, Tallahassee, FL 32306, United States.

Journal of leukocyte biology

|October 1, 2025

概括

脂聚糖 (LPS) 刺激导致巨细胞内先天免疫基因促进者的短暂重塑. 染色质敏感性变化发生在两个阶段,揭示了对免疫反应动态的时间洞察力.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

巨细胞利用类似收费的受体4 (TLR-4) 来检测脂多糖 (LPS),从而启动基因表达的信号级联.
在LPS刺激后的巨细胞中,早期和晚期的基因表达模式涉及初级和二级反应基因.
在LPS诱导的巨细胞激活期间,促进体区域中核细胞结构的动力学尚未完全理解.

研究的目的:

在LPS刺激后,研究巨细胞促进器区域中核细胞结构的时间动态.
了解染色体重塑如何影响免疫反应早期和晚期的基因表达.

主要方法:

用LPS刺激THP-1衍生的巨细胞.
在8个时间点对促进者进行核细胞分布和微球菌核酶 (MNase) 敏感度的评估.
分析的重点是初级和二级响应阶段的促进者地区.

主要成果:

核细胞分布在大多数促进体中保持不变,在一组天生的免疫基因促进体中观察到过渡性重塑.
显著的MNase敏感性改变发生在两个阶段,与早期和晚期的基因表达模式相关.
在RNA聚合酶II (Pol II) 促进剂中,无论转录变化如何,都注意到广泛的染色质敏感性改变.

结论:

激发LPS会诱导暂时促进体重塑和巨细胞中明显的染色质敏感性变化.
这些发现提供了对免疫刺激对促进器架构影响的时间见解.
该研究为其他生物系统的时间解析色素重塑研究提供了框架.

关键词:

这就是TLR-4信号.染色体重塑染色体重塑的方法巨细胞对LPS的反应核子组的动力学核细胞的敏感性核细胞的敏感性

更多相关视频

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

Bone Marrow-derived Macrophage Production

Bone Marrow-derived Macrophage Production

Published on: November 22, 2013

相关概念视频

Immune Surveillance by NK Cells and Phagocytes

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...

Cells of the Innate Immune Response

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...