Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Amperometry: Overview01:10

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Chiral-Induced Selective Sensing and Transport of Irinotecan by P-Glycoprotein-Inspired Nanochannel Sensors.

ACS sensors·2026
Same author

Recent Advances in Pesticide Nanocapsules from Chemical Design to Controlled Release.

Journal of agricultural and food chemistry·2026
Same author

Bioinspired hierarchical porous membrane for efficient enrichment and quantitative analysis of the environmental metabolite 3,5-dichlorosalicylic acid.

Talanta·2026
Same author

Supramolecular cucurbit[7]uril@AuNPs self-assembled membranes for impedimetric sensing and selective removal of cationic dyes.

Analytica chimica acta·2025
Same author

Recent Advances in Fluorescent Detection of Pesticides in Environmental and Food Matrices: From Molecular Probes to Nanoparticle-Based Sensors.

Journal of agricultural and food chemistry·2025
Same author

Super-amphiphilic adjuvants for enhancing the spreading and deposition performance of pesticide on corn leaves in high-temperature conditions.

Journal of colloid and interface science·2025

相关实验视频

Updated: Jun 30, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
13:57

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

30.2K

一个基于Calix[4]arene的湿度接口传感器,用于快速检测ATP.

Haonan Qu1, Haifan Zhang1, Cuiguang Ma1

  • 1State Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan, 430079, PR China.

Biosensors & bioelectronics
|October 1, 2025
PubMed
概括

研究人员开发了一种新型的二胺基 [4] 烯 (BAC4) 材料,用于检测腺三酸盐 (ATP),这是一种关键的细胞能量指标. 这一进步为生物医学和生物技术中的应用提供了精确的检测.

关键词:
这是一个ATP传感器.卡利克斯 (Calix) 是一个[4]arene 的生物.点击"化学"就可以了.湿透性 湿透性 湿透性

更多相关视频

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
10:00

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

Published on: October 12, 2015

12.3K
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.2K

相关实验视频

Last Updated: Jun 30, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
13:57

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

30.2K
Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
10:00

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

Published on: October 12, 2015

12.3K
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.2K

科学领域:

  • 生物化学 生物化学
  • 材料科学 材料科学 材料科学
  • 纳米技术纳米技术

背景情况:

  • 腺三酸盐 (ATP) 是细胞能量代谢的关键指标.
  • ATP检测技术对于生物医学,微生物学,生物工程,精密医学和生物技术的进步至关重要.

研究的目的:

  • 为了合成和表征一种新型的二胺基 [4] 烯 (BAC4) 材料.
  • 开发使用BAC4-修改自组装单层 (BAC4-SAM) 的ATP表面检测平台.
  • 通过BAC4-SAM研究ATP识别的选择性,敏感性和机制.

主要方法:

  • 通过点击化学合成二胺基 [4] 烯 (BAC4) 并通过点击化学对其表面进行修改.
  • 使用X射线光电子光谱 (XPS) 和水接触角度 (CA) 进行BAC4-SAM的表征.
  • 对ADP和AMP的ATP检测选择性的评估,以及降至5.5×10−6M的灵敏度.
  • 使用UV-vis,1H NMR和AFM对识别机制的分析.
  • 使用开发的BAC4-SAMs平台监测ATP水解.

主要成果:

  • 在85%的产量中合成了BAC4,并在表面上成功修改为BAC4-SAM.
  • BAC4-SAM对ATP比ADP和AMP具有很高的选择性.
  • 在ATP的存在/缺席下,BAC4-SAM的可湿性在超性和超性状态之间被可逆调节.
  • 在低至5.5 × 10−6 M的度下,ATP是可以检测到的.
  • 识别机制得到了阐明,并成功实现了ATP水解监测.

结论:

  • 开发的BAC4-SAM平台为ATP检测提供了一种敏感和选择性的方法.
  • 可逆的湿度变化提供了ATP存在的视觉指标.
  • 这项技术在需要ATP监测的各个领域都有潜在的应用,包括诊断和生物技术.