在RSV感染后儿童和成人显著的微生物群变异及其与宿主反应的关联
Qi Wang1, Yulin Zhang2, Xiaoxuan Yao3
1Department of Pulmonary and Critical Care Medicine, Second Affiliated Hospital of Harbin Medical University, Harbin Medical University, 246 Xuefu Road, Harbin, 150086, China; National Center for Respiratory Medicine; State Key Laboratory of Respiratory Health and Multimorbidity; National Clinical Research Center for Respiratory Diseases; Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, Laboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, 2 Yinghua Dongjie, Beijing, 100029, China.
与成年人相比,儿科呼吸道同胞性病毒 (RSV) 患者表现出明显的喉微生物组变化和免疫反应. 呼吸道微生物群中的这些差异可能解释了为什么儿童会经历更严重的RSV疾病.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 儿科 儿科 儿科
背景情况:
- 呼吸道同胞性病毒 (RSV) 在儿童中比成年人更严重地引起疾病.
- 呼吸道微生物群组成的差异可能是这种差异的基础.
- 在儿科RSV中,喉微生物组与宿主免疫反应之间的相互作用尚未得到充分理解.
研究的目的:
- 调查成人和儿科RSV患者喉微生物组的变化.
- 探索这些微生物组变化与宿主免疫反应之间的关系.
- 确定导致儿童RSV病原性增加的因素.
主要方法:
- 16S rRNA基因测序和喉抽样的转录组分析.
- 在RSV患者 (住院患者和门诊患者),社区获得性肺炎 (CAP) 患者和健康对照者中比较喉微生物组和基因表达特征.
- 微生物丰富度与宿主基因表达之间的相关性分析.
主要成果:
- 儿科RSV住院患者的口服无气体丰度 (Prevotella,Veillonella) 比门诊患者高.
- 在儿科住院患者和门诊患者中发现了明显的喉微生物组差异,但在成人中没有发现.
- 儿科住院患者与成年人相比,表现出更多与中性粒细胞通路相关的基因差异表达.
- 特定的细菌群 (Alphaproteobacteria,Actinobacteria) 与儿科住院患者的炎症基因表达 (CXCL10,CXCL11) 相相关.
结论:
- 喉微生物组组成和宿主免疫反应在儿科RSV住院患者和门诊患者之间存在显著差异.
- 这些宿主微生物群相互作用,特别是涉及中性粒细胞的招募,可能会导致儿童严重的RSV疾病.
- 研究结果提供了关于RSV在儿科群体中的致病性增加的见解.
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