来自蒸汽爆炸的米,结合紫外线-A辐射的乙醇溶性lignin的杀菌作用
Akihiro Shirai1, Akihiro Suzuki2, Yoshitoshi Nakamura3
1Department of Bioscience and Bioindustry, Graduate School of Technology, Industrial and Social Sciences, Tokushima University.
Journal of microorganism control
|October 1, 2025
概括
草中的乙醇溶性红素,当与紫外线A (UV-A) 光相结合时,可以有效地杀死细菌. 这种抗菌作用是由过程中产生的活性氧物种 (ROS) 介导的.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 微生物学 微生物学
背景情况:
- 生物质加工的副产品素具有潜在的抗微生物特性.
- 研究素的新型应用,如抗菌剂,对于可持续实践至关重要.
研究的目的:
- 为了评估来自米的乙醇可溶性红素 (ESL) 的杀菌效果.
- 确定ESL与紫外线-A (UV-A) 辐射相结合的作用机制,以对抗细菌.
主要方法:
- 米草经过蒸汽爆炸预处理,随后进行乙醇提取以获得ESL.
- 在UV-A照射下测试了杀菌活性对抗格拉姆阳性和格拉姆阴性细菌.
- 使用光显微镜和扫描电子显微镜观察细菌细胞与ESL的相互作用.
- 分析了酸染色,细胞内ATP水平和反应性氧物种 (ROS) 生成.
主要成果:
- 在UV-A照射下,ESL在低度 (≤10毫克TPC/L) 的细菌中表现出显著的杀菌活性.
- ESL吸附在细菌细胞表面上,导致膜变形和透性增加.
- 对ESL的UV-A辐射产生过氧化,而ROS吸尘器抑制了杀菌效应,证实了ROS的参与.
结论:
- 来自大米的乙醇溶性红素与UV-A辐射具有强大的协同抗菌活性.
- 杀菌机制涉及ROS生成,导致细菌细胞损伤和死亡.
- 这项研究表明,有可能开发与UV-A技术相结合的基于素的抗微生物应用.
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