H2O2介导的酸盐饥饿反应的氧化促进了适应米低酸盐的情况
Funing Meng1, Dan Xiang1,2, Ziqi Bu3
1State Key Laboratory of Plant Environmental Resilience, College of Life Sciences, Zhejiang University, Hangzhou, China.
Nature communications
|October 1, 2025
概括
由OsRBOH-D/H产生的过氧化 (H2O2) 在低酸盐条件下有助于米的酸盐吸收. 这种H2O2激活了OsPHR2转录因子,增强了植物适应酸盐饥饿的能力.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- (P) 是植物生长的重要宏观营养素,主要以无机 (Pi) 的形式被吸收.
- 植物拥有复杂的Pi饥饿反应 (PSR) 机制,以应对低Pi (LP) 条件.
- 过氧化 (H2O2) 是植物应激反应中已知的信号分子,但其在PSR中的作用尚不清楚.
研究的目的:
- 调查H2O2在米Pi饥饿反应中的作用.
- 阐明H2O2在LP压力下影响酸盐吸收和利用的分子机制.
主要方法:
- 在低酸盐条件下对大米中H2O2生产的分析.
- 研究H2O2对酸盐吸收和利用的影响.
- 分子和生化分析以确定H2O2与OsPHR2转录因子之间的相互作用.
- 基因表达分析以确定OsRBOH-D作为OsPHR2.2的目标.
主要成果:
- 由OsRBOH-D/H产生的低酸盐诱导的H2O2增强了酸盐的吸收和利用.
- H2O2在Cys377氧化OsPHR2,促进其寡合化,DNA结合和核转位,从而激活PSR.
- OsRBOH-D被确定为OsPHR2的直接点基因,建立了一个积极的反循环.
结论:
- H2O2在米Pi饥饿反应中起到关键的第二信使作用,放大信号通路.
- 由H2O2介导的OsPHR2-OsRBOH-D调节循环对于大米适应低酸盐环境至关重要.
- 这项研究揭示了一种涉及H2O2在植物营养信号和应激适应中的新机制.
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