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RNA-的相互作用调整了协同的微滴分散体内的 ribozyme 活性

Basusree Ghosh¹, Patrick M McCall^1,2,3,4,5, Kristian Kyle Le Vay⁶

¹Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

Nature communications

|October 1, 2025

概括

从RNA和中形成的无膜共体液滴可以分隔生物分子. 序列的变化显著改变了这些液滴中的 ribozyme 活性,为早期生命进化提供了洞察力.

科学领域:

生物化学生物化学
生命的起源研究生命的起源研究
分子生物学分子生物学

背景情况:

无膜复杂的同体微滴可以作为原始细胞区的模型.
了解分子相互作用对同体特性和催化活性的影响,对于生命起源研究和现代生物学至关重要.

研究的目的:

为了研究一个最小的头 ribozyme 的协体组成,物理化学性质和催化活性之间的关系.
探索序列的变化如何影响RNA-共聚微滴中的 ribozyme 功能.

主要方法:

用益生菌相关的氨基酸和头 ribozyme 制备RNA-的同微粒.
检测协同体滴中的 ribozyme 催化活性,并与缓冲条件进行比较.
对 ribozyme 度,扩散系数和催化速率常数之间的相关性进行分析.

主要成果:

与缓冲溶液相比,Ribozyme的催化活性被发现被抑制在协同生菌微环境中.
序列的调节导致 ribozyme 反应速率和产量多达 15 倍的变化.
рибо酶的表面速率常数与其度呈反相关性,与其扩散系数呈直接相关性.

结论:

协同生微环境的物理化学特性显著影响封装的 ribozyme 催化活性.
没有膜的隔间可以施加选择压力,这可能会推动分子进化,正如在前生物场景中观察到的.
这项研究提供了一个框架,以了解同体结构和生化功能之间的相互作用.

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