在癌细胞中使用大气压电离技术对癌细胞进行醇分析
Pia Wittenhofer1, Juan F Ayala-Cabrera1,2, Laila Orell1
1Applied Analytical Chemistry, University of Duisburg-Essen, Universitaetsstrasse 5, 45141, Essen, Germany.
Analytical and bioanalytical chemistry
|October 1, 2025
概括
一个新的管状等离子电离 (TPI) 源通过LC-MS提供了灵敏而强大的类固醇分析,优于电喷射电离和匹配大气压化学电离在复杂的生物样本中.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 质谱测量质量谱测量
背景情况:
- 固醇生物合成分析面临诸如广泛度范围和导致离子抑制的矩阵干扰等挑战.
- 液体染色学-质谱学 (LC-MS) 是至关重要的,但敏感的检测严重依赖于有效的电离技术.
研究的目的:
- 开发和评估一种用于LC-MS醇分析的新型管血电离源 (TPI).
- 为了比较TPI与已建立的电喷离子化 (ESI) 和大气压化学离子化 (APCI) 方法的性能.
主要方法:
- 为LC-MS开发一个新的管体等离子离子源.
- 对TPI,ESI和APCI进行比较分析,以测量固醇电离效率和灵敏度.
- 将TPI源应用于复杂的生物矩阵:人体血,HepG2细胞和肝脏组织.
主要成果:
- 与APCI相比,TPI来源显示了可比的量化极限 (LOQ) 和与ESI相比更高的灵敏度.
- 在长时间的测量过程中,TPI和APCI提供了稳定的信号,与ESI不同,ESI遭受了显著的离子抑制.
- 使用TPI测量醇的结果与所有测试的生物样本中使用APCI获得的结果非常相似.
结论:
- 新型TPI源是复杂生物矩阵中固醇分析的灵敏和强大的工具.
- TPI为ESI和APCI提供了一个有希望的替代方案,用于挑战固醇量化工作流程.
- 这一进步有助于更可靠地分析固醇生物合成和相关的代谢途径.
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