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单细胞和空间转录组学的综合多组学分析揭示了与hpv相关的独特免疫微环境特征和宫癌的预后特征

Qiuyue Su¹, Xiangdong Tian², Fucheng Li³

¹Department of Gynecological Radiotherapy, Harbin Medical University Cancer Hospital, Harbin, China.

Frontiers in immunology

|October 2, 2025

概括

这项研究使用先进的测序揭示了HPV阳性与HPV阴性宫癌 (CC) 中不同的免疫微环境. 我们确定了关键的细胞相互作用,并为精确的CC治疗开发了预后特征.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
基因组学就是基因组学.

背景情况:

宫癌 (CC) 的异质性,特别是HPV阳性和HPV阴性亚型之间的免疫微环境空间差异,尚不清楚.
持续高风险的人类乳头瘤病毒 (HPV) 感染是CC的主要驱动因素,影响其多样性.

研究的目的:

在HPV阳性和HPV阴性CC中全面分析免疫微环境的空间异质性.
识别不同的细胞亚型,它们的空间分布和细胞间通信通路.
根据免疫微环境特征开发CC的预后签名.

主要方法:

集成的单细胞RNA测序 (scRNA-seq),空间转录组学 (ST) 和大量RNA-seq.
多重免疫光对HPV状态特定表达的验证.
考克斯和LASSO对TCGA数据集的回归分析用于预后签名识别.

主要成果:

阳性CC显示CD4+T细胞和cDC2s的增加; 阴性CC显示CD8+T细胞的透增加.
不同的途径 (例如,HPV阳性的ANXA1-FPR1 / 3,HPV阴性的单细胞/巨细胞相互作用) 控制免疫细胞调节.
开发了一种与上皮细胞相关的新型特征 (ERS),与CC患者的存活率和免疫微环境变异有显著的相关性.

结论:

综合多omics数据精确定义了空间转录特征和基于HPV状态的CC中的免疫细胞通信.
确定了预后性ERS,与免疫环境和宫癌患者的结果密切相关.
这些发现提高了对CC分子机制的理解,并支持精确的治疗策略.

关键词:

在HPV中,HPV是HPV.宫癌:子宫癌是一种癌症.预后因素是一个预后因素.一个单细胞测序.空间转录学空间转录学瘤微环境是一个微环境.

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