维斯法促进细胞癌进展:从临床样本和细胞系模型中获得的证据
Eiji Kashiwagi1,2, Miho Ushijima1, Shohei Ueda1
1Department of Urology, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan.
Journal of kidney cancer and VHL
|October 2, 2025
概括
维斯法丁 (Visfatin) 是脏脂肪中的一种阿迪波金,促进细胞癌 (RCC) 的生长和恶性瘤. 抑制维斯法廷抑制了RCC细胞的增殖,这表明它是癌的治疗标.
科学领域:
- 在瘤学瘤学.
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
背景情况:
- 周周脂肪分泌阿迪波金,影响瘤发育,包括细胞癌 (RCC).
- 一种阿迪波金 (adipokine) 的Visfatin与RCC有关,但其在瘤进展中的具体作用尚不清楚.
研究的目的:
- 为了研究RCC患者周围脂肪中的visfatin表达.
- 为了将维斯法廷水平与RCC恶性病变相关联.
- 在试管体内检查visfatin在RCC细胞系中的作用.
主要方法:
- 实时PCR测量57名RCC患者脂肪组织中的维斯法丁mRNA.
- 在人体RCC细胞系上进行体外MTT测定和西方斑点.
- 评估了维斯法添加剂和FK866 (维斯法抑制剂) 对细胞增殖和蛋白质表达的作用.
主要成果:
- 周围瘤脂肪中较高的visfatin mRNA表达与较高的Fuhrman等级 (≥2) RCC相关.
- 此外,Visfatin促进了RCC细胞的增殖,增加了HIF1α,p-Akt和p-ERK.
- FK866抑制了RCC细胞的增殖,并降低了这些蛋白质水平.
结论:
- 周围瘤脂肪组织衍生维斯法因影响RCC恶性并促进瘤生长.
- 维斯法丁可能是干预RCC进展的治疗点.
- 脂肪组织通过涉及visfatin的机制促进RCC的进展.
更多相关视频
11:27Modeling Spontaneous Metastatic Renal Cell Carcinoma mRCC in Mice Following Nephrectomy
Published on: April 29, 2014
17.2K
09:29Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
Published on: September 30, 2016
14.3K
相关概念视频
03:03mTOR Signaling and Cancer Progression
4.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.6K
02:07Tumor Progression
7.2K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.2K
02:38Mitogens and the Cell Cycle
7.7K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.7K