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基于使用SPR的全转录因子 (aTF) 的四环素和红素的敏感定量检测技术

Yingzi Wang¹, Chen Zhao¹, Shang Wang¹

¹Military Medical Sciences Academy, Academy of Military Sciences, Tianjin 300050, China. tianjinszq922@sohu.com.

Analytical methods : advancing methods and applications

|October 2, 2025

概括

这项研究开发了一种使用全转录因子 (aTF) 检测抗生素残留物,如四环素和红素的敏感生物传感器. 该方法提供了准确的环境监测,以预防耐药细菌.

科学领域:

生物化学生物化学
环境科学环境科学
分析化学分析化学

背景情况:

农业中的抗生素残留物带来了耐药细菌和生态系统破坏的风险.
敏感的检测技术对于监测环境抗生素污染至关重要.
所有的转录因子 (aTF) 为生物传感器的发展提供了高的特异性和灵敏性.

研究的目的:

开发一种使用全转录因子检测抗生素残留的敏感检测方法.
通过测量联体诱导的DNA结合亲和力变化,量化分析四环素和红色素度.
建立适用于环境监测和质量控制的强大的生物传感器.

主要方法:

利用了全转录因子 (TetR和MphR),这些因子在结合联体时改变了DNA亲和力.
采用表面等离子共振 (SPR) 技术来测量DNA结合前后的亲和力变化.
优化样品化和系统注射时间,以提高检测.

主要成果:

通过基于SPR的亲和度测量实现了对环素和红色素的定量检测.
在优化条件下观察到两个数量级的亲和力变化.
确定了低检测极限:四环素0.8nM,红色素1nM.

结论:

开发的SPR检测方法对四环素和红素具有高度敏感性和准确性.
这种生物传感器技术对环境监测和质量控制应用具有重大前景.
基于aTFs的SPR检测为解决抗生素残留问题提供了有价值的工具.

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