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组装一个高质量的Polypterus senegalus双胞胎基因组

Jeong Woen Shin¹, Bo-Mi Kim², Jun Kim^3,4

¹Department of Bio-AI Convergence, Chungnam National University, 99, Daehak-Ro, Yuseong-Gu, Daejeon, 34134, Republic of Korea.

Genes & genomics

|October 2, 2025

概括

这项研究产生了Polypterus senegalus的高质量基因组组,揭示了对脊椎动物适应和器官进化至关重要的基因表达模式. 这些发现为未来的进化研究提供了宝贵的基因组资源.

科学领域:

进化生物学进化生物学
基因组学就是基因组学.
进行比较基因组学.

背景情况:

塞内加勒斯鱼 (Polypterus senegalus) 是一种关键的鱼类模型,用于了解脊椎动物的陆地适应.
测序方面的进步需要更新P. senegalus.的基因组资源.

研究的目的:

为P. senegalus. 构建高质量的部分分相基因组组件.
为了利用高保真度的长读测序数据来改进基因组组装.

主要方法:

使用PacBio HiFi测序数据生成了部分分相P. senegalus基因组组件.
根据长度,连续性,准确性和BUSCO完整性,将新组合与以前的基因组组合进行比较.
解决了重复区域和确定了端粒到端粒染色体.

主要成果:

与以前的基因组相比,新的组件显示出更高的质量.
使用集成的短读和长读RNA测序,注释了20940个基因.
转录学揭示了与空气呼吸,血管生成和四肢灵活性相关的组织特异性基因表达.
在肺和游泳膀中确定了Tcf21,Foxf1和Tbx3a的协调表达,这表明它们具有共同的发育起源.
在肺,脏和脏之间发现了生理上的相似之处.

结论:

这项研究为P. senegalus提供了宝贵的基因组资源.
提供了关于脊椎动物器官进化和适应的分子机制的新见解.

关键词:

多鸟塞内加卢斯 (Polypterus senegalus) 是一个比较古老的动物.基因表达基因表达基因表达基因组组装组的基因组组装组高准确度的长读序列测序.部分阶段化基因组的基因组.

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